Association between cytokines and exosomes in synovial fluid of individuals with knee osteoarthritis

Modern Rheumatology
Kun GaoYong Jiang

Abstract

Background: Cytokines in synovial fluid (SF) play a crucial role in knee osteoarthritis (KOA). Exosomes are nanovesicles that are abundant in SF and carry a large quantity of signaling molecules. The purpose of this study was to evaluate the cytokine profiles of SF-derived exosomes and try to explore its biological function.Methods: Twenty-four KOA patients who were scheduled for their first intra-articular injection or knee replacement surgery were enrolled and divided into the KL1-2 group and the KL3-4 group according to the Kellgren and Lawrence (KL) classification. SF was collected from the patient's knee for the isolation of exosomes. A multiplex cytokine assay was performed to detect the 21 cytokines in the exosomes. The SF derived-exosomes were exposed to PBMCs and chondrocytes to assess their immunomodulatory potential.Results: Exosomes were successfully extracted from the SF, with an average diameter of 92 nm. Most cytokines were detectable in the SF-derived exosomes. Twelve inflammatory cytokines and eight chemokines were elevated in the exosomes of the KL3-4 group compared to that of the KL1-2 group (p < .05). A higher number of PBMCs were chemo attracted and the proliferation of chondrocytes was restrained by the SF...Continue Reading

References

Nov 23, 2000·Annals of the Rheumatic Diseases·F HéraudM F Harmand
Nov 30, 2000·Arthritis Research·R W KinneG R Burmester
Dec 17, 2002·Arthritis and Rheumatism·Rosa Maria BorziAndrea Facchini
Oct 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Denise MerzRobert Terkeltaub
Jan 6, 2006·Scandinavian Journal of Rheumatology·C E VergunstP P Tak
Jun 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Huang-Ge ZhangRobert P Kimberly
Mar 6, 2012·Bone·Carla R Scanzello, Steven R Goldring
Feb 8, 2014·Canadian Journal of Physiology and Pharmacology·Zhiqiang YeDaozhang Cai
May 31, 2014·Mediators of Inflammation·Piotr WojdasiewiczDariusz Szukiewicz
Dec 17, 2014·The Journal of Infectious Diseases·Kateena Addae KonaduVincent C Bond
Aug 10, 2017·Frontiers in Immunology·Stinne R GreisenBent Deleuran
Oct 17, 2017·Current Opinion in Rheumatology·Shigeru Miyaki, Martin K Lotz
Jul 3, 2018·Journal of Extracellular Vesicles·Andrew D FoersKen C Pang
Jul 4, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zeng LiXisheng Weng
Nov 30, 2018·Frontiers in Immunology·Janneke BoereMarca H M Wauben
Jan 8, 2019·The Journal of Arthroplasty·Ronald E DelanoisMichael A Mont
Jan 18, 2019·Pain Research & Management : the Journal of the Canadian Pain Society = Journal De La Société Canadienne Pour Le Traitement De La Douleur·Dong HuangJin-Feng Liu

❮ Previous
Next ❯

Citations

Nov 15, 2019·Current Pharmaceutical Design·Chao TuZhihong Li
Nov 30, 2019·BioMed Research International·Cheng JuZhiping Zhang
Jun 9, 2020·Oxidative Medicine and Cellular Longevity·Carlos Rocha Oliveira, Rodolfo Paula Vieira
Jul 1, 2020·Bone Research·Zhenhong NiLin Chen
Mar 9, 2021·Stem Cells International·Qian JinWenrong Xu
Jun 20, 2021·Bone Research·Zhenhong NiLin Chen
Aug 8, 2021·International Journal of Molecular Sciences·Xin ZhangVirginia Byers Kraus
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jianjing LinQiang Liu
Oct 26, 2021·Frontiers in Immunology·Xin ZhangVirginia B Kraus
Dec 11, 2021·Frontiers in Pharmacology·Jinxia DengBo Wei

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Yun ZhouGuohui Yang
Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Yusong ZhangGang Wang
Chinese Medical Sciences Journal = Chung-kuo I Hsüeh K'o Hsüeh Tsa Chih
Haibo SiBin Shen
© 2021 Meta ULC. All rights reserved