Association between incubation time and genotype in sheep experimentally inoculated with scrapie-positive brain homogenate

American Journal of Veterinary Research
Marie S BulginMary E Matlock

Abstract

To compare incubation time and clinical signs of scrapie in codon 136/171 alanine-valine/glutamine-glutamine (AVQQ) experimentally inoculated sheep with that in sheep with the more common 136/171 AAQQ genotype. 60 Suffolk sheep. Twenty-seven 171 QQ ewes purchased from 2 private flocks were bred with a 171 QQ Suffolk ram before being inoculated with a 20% solution of scrapie-positive brain homogenate (5 mL, PO) from sheep containing genotypes 136/154/171 AA/arginine-arginine (RR)/QQ, AVRRQQ, and VVRRQQ that had died of scrapie. Ewes had 33 lambs, which were inoculated in the same manner on the day of birth. All 16 genotype 136/154/171 AVRRQQ sheep that died of scrapie were 9 to 11 months of age; clinical signs lasted 1 day to 3 weeks with no wasting and only mild pruritus. The first AARRQQ sheep died with typical clinical signs of scrapie 27 months after inoculation, and 14 were still alive 37 to 42 months after inoculation. The 136/171 AVQQ sheep had minimal accumulation of modified cellular protein (PrP(SC)) as determined by immunohistochemical (IHC) staining within affected cells; thus the severity of clinical signs and time of death were not associated with brain lesions or the amount of PrP(SC) in brain TISSUE OF 136/154/17...Continue Reading

References

Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W GoldmannJ Hope
Aug 6, 1988·The Veterinary Record·J D Foster, A G Dickinson
Nov 1, 1982·The Journal of Infectious Diseases·W J HadlowR E Race
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·L ManuelidisW Fritch
Jun 6, 1994·Annals of the New York Academy of Sciences·N HunterJ Hope
Jul 1, 1993·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J M MillerR E Race
Jun 5, 1998·The Veterinary Record·K I O'RourkeD P Knowles
Jun 12, 2001·Journal of Neuropathology and Experimental Neurology·F O Bastian, J W Foster
Dec 13, 2002·Journal of Virology·Jason C BartzRichard A Bessen
Jun 28, 2003·Journal of Virology·Alana M ThackrayRaymond Bujdoso
Feb 19, 2004·Cytogenetic and Genome Research·U DeSilvaB A Roe
Aug 11, 2004·The Journal of General Virology·M BaylisM B Gravenor
Aug 25, 2004·Medical Hypotheses·L Broxmeyer
Sep 10, 2004·Current Molecular Medicine·Joaquin CastillaClaudio Soto
Oct 16, 2004·The Journal of General Virology·J-Y MadecT Baron
Sep 22, 2005·American Journal of Veterinary Research·Jessica M EvoniukDale A Redmer

❮ Previous
Next ❯

Citations

May 16, 2007·Journal of the American Veterinary Medical Association·Marie S Bulgin, Sharon Sorensen Melson
Apr 2, 2010·The Journal of General Virology·Richard RubensteinMichael W Miller
Feb 11, 2012·The Journal of General Virology·Richard RubensteinGiuseppe LaFauci
Mar 29, 2012·Prion·Gültekin TamgüneyStanley B Prusiner
Jul 17, 2009·Veterinary Pathology·A N HamirR G Rohwer
Dec 30, 2008·Veterinary Pathology·A N HamirJ M Miller

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cachexia & Brown Fat

Cachexia is a condition associated with progressive weight loss due to severe illness. In cancer patients, it is proposed to occur as a result of tumor-induced energy wasting. Several proteins have been implicated in browning and depletion of white adipose tissue. Here is the latest research on cachexia and brown fat.

Cardiac Cachexia

Cardiac cachexia is a syndrome associated with the progressive loss of muscle and fat mass. It most commonly affects patients with heart failure and can significantly decrease the quality of life and survival in these patients. Here is the latest research on cardiac cachexia.

Related Papers

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Amir N HamirAllen L Jenny
Journal of the American Veterinary Medical Association
Marie S Bulgin, Sharon Sorensen Melson
© 2021 Meta ULC. All rights reserved