Association between integrin-dependent migration capacity of neural stem cells in vitro and anatomical repair following transplantation

Molecular and Cellular Neurosciences
L PrestozC ffrench-Constant

Abstract

In previous transplantation studies using neural stem cell lines immortalized by the temperature-sensitive SV40 large T-antigen, we have shown that animals with experimental hippocampal lesions resulting from four vessel occlusion recover spatial memory functions more effectively when grafted with the MHP36 cell line than with the MHP15 cell line [Gray et al. (1999). Philos. Trans. R. Soc. London Biol. Sci. 354:1407-1421]. In the present study, we have investigated the cellular and molecular basis of these differences in repair capacity both in vivo and in vitro. Using the same model of hippocampal damage we have shown that following transplantation MHP36 cells migrate and align within the damaged CA1 of the ipsilateral hippocampus. MHP15 cells, in contrast, migrate in a more indiscriminate pattern that does not reflect the anatomy of the region. To analyze the migratory properties of these two cell lines in more detail, we performed migration assays at a nonpermissive temperature on the extracellular matrix substrates laminin, fibronectin, and vitronectin. These showed that MHP36 cells have a greater migration potential than the MHP15 cells. While the pattern of cell surface extracellular matrix receptors of the integrin famil...Continue Reading

References

Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P S JatD Kioussis
Mar 1, 1995·Journal of Neuropathology and Experimental Neurology·R A SobelJ R Hinojoza
Apr 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Bronner-Fraser
Feb 16, 1996·Science·C LoisA Alvarez-Buylla
Feb 9, 1996·Cell·D A Lauffenburger, A F Horwitz
Apr 4, 1997·Science·R McKay
May 1, 1997·Neuron·H WichterleA Alvarez-Buylla
Sep 22, 1998·Current Biology : CB·E Georges-LabouesseA Gansmüller
Jan 23, 1999·Biology of Reproduction·J A Bowen, J S Hunt
Jul 15, 1999·Archives of Neurology·M F Mehler, J A Kessler
Oct 9, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J A GrayJ Sinden
Oct 26, 1999·Molecular Medicine Today·L S ShihabuddinF H Gage
Feb 19, 2000·Current Opinion in Cell Biology·D R Critchley
May 10, 2000·The Journal of Biological Chemistry·E F PlowJ W Smith
Sep 6, 2000·Trends in Genetics : TIG·A De Arcangelis, E Georges-Labouesse

❮ Previous
Next ❯

Citations

Sep 10, 2011·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Tamir Ben-Hur
Dec 17, 2009·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jens BenninghoffAngelo Luigi Vescovi
May 4, 2005·Current Neurology and Neuroscience Reports·Louis N Manganas, Mirjana Maletic-Savatic
Nov 8, 2008·Annals of the New York Academy of Sciences·Tamir Ben-Hur, Steven A Goldman
Sep 2, 2008·Journal of Cellular Biochemistry·Sujoy K Dhara, Steven L Stice
Apr 27, 2005·Brain Research. Brain Research Reviews·Stefano PluchinoGianvito Martino
Dec 8, 2004·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·M BrodhunS Patt
Jun 2, 2009·Journal of Pharmacological Sciences·Noritaka NakamichiYukio Yoneda
Nov 7, 2012·Cell Transplantation·Dah-Ching DingShinn-Zong Lin
May 29, 2004·Current Opinion in Neurology·Stefano PluchinoGianvito Martino
Sep 5, 2006·NMR in Biomedicine·Cecilie BrekkeMichel Modo
Nov 21, 2007·Bioprocess and Biosystems Engineering·Yuen Ling Ng, Howard A Chase
May 10, 2008·Minimally Invasive Therapy & Allied Technologies : MITAT : Official Journal of the Society for Minimally Invasive Therapy·Marc Maegele, Ute Schaefer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.