PMID: 8947469Nov 15, 1996Paper

Association between phosphatidylinositol-3 kinase, Cbl and other tyrosine phosphorylated proteins in colony-stimulating factor-1-stimulated macrophages

The Biochemical Journal
V KanagasundaramJ A Hamilton

Abstract

Colony stimulating factor-1 (CSF-1) stimulation of the macrophage cell line BAC1.2F5 and murine bone marrow-derived macrophages resulted in tyrosine phosphorylation of phosphatidylinositol-3 kinase (PI-3 kinase) p85 alpha and its stable association with several tyrosine phosphorylated proteins, including CSF-1 receptor (p165), p120, p95 and p55-p60. p120 co-migrated with the product of the protooncogene c-cb1 in anti-p85 alpha immunoprecipitates, and associated with p85 alpha in a rapid and transient manner. Reciprocal experiments confirmed the presence of p85 alpha in anti-Cb1 immunoprecipitates on CSF-1 stimulation of macrophages. PI-3 kinase immunoprecipitates from the myeloid FDC-P1 cell line expressing mutant CSF-1 receptor (Y721F), which does not associate with PI-3 kinase, still contained Cbl. The identity of the tyrosine phosphorylated protein p95 remains unknown. The interaction between p85 alpha and the tyrosine phosphorylated proteins survived anion-exchange chromatography, suggesting perhaps the presence of a stable complex; furthermore, in CSF-1-treated BAC1.2F5 cell extracts, only one of the two pools of PI-3 kinase separated by chromatography was present in this putative complex. The association did not appear to...Continue Reading

Citations

Dec 10, 1999·The Journal of Biological Chemistry·K L BergE R Stanley
Sep 11, 2003·Molecular & Cellular Proteomics : MCP·Yee-Guide Yeung, E Richard Stanley
Jun 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·John RoiniotisJohn A Hamilton
Nov 4, 2005·Proteomics·Maddalena CrossJohn A Hamilton
Oct 19, 2001·Oncogene·A Y TsygankovG Swaminathan
Oct 12, 2000·The Journal of Biological Chemistry·G Pedraza-AlvaY Rosenstein
Aug 28, 1998·Cellular Signalling·Y C Liu, A Altman
Jul 1, 1997·Immunology Today·J A Hamilton
Jun 3, 1998·The International Journal of Biochemistry & Cell Biology·J A HamiltonA Jaworowski
Mar 15, 2002·European Journal of Cell Biology·Frederic BardRoland Baron

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