Association between rate of viral genome replication and virulence of Marek's disease herpesvirus strains

Virology
R YunisK A Schat

Abstract

The early pathogenesis of Marek's disease virus (MDV) infection is characterized by a lytic infection followed by the induction of latency. Genetically resistant N2a and susceptible P2a chickens were infected with the less virulent JM-16 or the very virulent plus (vv+) RK-1 MDV strains to examine the relationship between virulence and resistance on virus replication during 1-10 days postinfection (dpi) using real-time quantitative polymerase chain reaction (qPCR) and quantitative reverse transcriptase (qRT)-PCR assays. The numbers of copies of the viral DNA or transcripts amplified by these assays were normalized relative to cellular controls and subjected to three-way ANOVA. Viral DNA but not RNA was present in spleens at 1-3 dpi in decreasing quantities, and at 4 dpi, viral DNA started to increase concomitant with the initiation of viral transcription independently of virus strain and genetic resistance. At 6 dpi, JM-16 became latent in resistant N2a and susceptible P2a chickens with low levels of viral transcripts, but transcriptional activity increased in susceptible P2a chickens at 9 and 10 dpi. In contrast, infection with vv+ RK-1 never went into latency in both chicken lines. Viral transcripts were present from 4 to 10 d...Continue Reading

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Citations

Jul 25, 2014·Avian Diseases·John R DunnCelina Buscaglia
Sep 6, 2005·Journal of Virology·Keith W JarosinskiKarel A Schat
Jul 20, 2007·Journal of Virology·Keith W JarosinskiNikolaus Osterrieder
Sep 30, 2005·Avian Pathology : Journal of the W.V.P.A·R L WitterK A Schat
Jun 19, 2012·Avian Pathology : Journal of the W.V.P.A·John R DunnRichard L Witter
Aug 28, 2012·Virus Genes·Stephen J SpatzRichard L Witter
Dec 2, 2008·Developmental and Comparative Immunology·Mohamed Faizal Abdul-CareemShayan Sharif
Nov 23, 2005·Journal of Virological Methods·Mohamed Faizal Abdul-CareemShayan Sharif
Jun 4, 2016·The Veterinary Journal·K JonesM Read
Dec 3, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Caixia GaoLiandong Qu
Nov 15, 2011·BMC Veterinary Research·Katherine E AtkinsMark E J Woolhouse
Oct 20, 2018·Avian Diseases·I M Gimeno, K A Schat
Oct 18, 2005·The Journal of General Virology·Susan J BaigentVenugopal K Nair
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Justin R PerraultAnnie Page-Karjian

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