Association between the vaginal microbiome and high-risk human papillomavirus infection in pregnant Chinese women

BMC Infectious Diseases
Yulian ChenWen Di

Abstract

In this study, the association between high-risk human papillomavirus (hrHPV) infection and the vaginal microbiome in pregnant women was evaluated in Chinese cohorts. The vaginal bacterial composition of four groups, 38 hrHPV-infected pregnant women (PHR, n = 38), pregnant women without HPV infection (PN, n = 48), nonpregnant women with hrHPV infection (NPHR, n = 19) and nonpregnant women without HPV infection (NPN, n = 30), was characterized by deep sequencing of barcoded 16S rRNA gene fragments (V3-4) using Illumina MiSeq. The results revealed that both pregnancy and HPV infection can increase vaginal bacterial microbial richness and diversity, with the bacterial composition being most influenced by pregnancy. Lactobacillus was the most dominant genus among all samples. NPN samples were dominated by CST (community state type) III, mainly composed of Lactobacillus iners. Both pregnancy and hrHPV infection were accompanied by an increased proportion of CST I (dominated by Lactobacillus crispatus), as opposed to CST III. Bifidobacterium, Bacillus, Megasphaera, Sneathia, Prevotella, Gardnerella, Fastidiosipila and Dialister were found to be biomarkers for hrHPV-infected women, though different genera (Bifidobacterium, Megasphaera...Continue Reading

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Citations

Apr 2, 2020·Frontiers in Immunology·Salwan Al-NasiryBoris W Kramer
Jul 14, 2020·Frontiers in Cellular and Infection Microbiology·Qian YangWeiguo Lu
Nov 17, 2020·Frontiers in Cellular and Infection Microbiology·Nicole K DinsdaleGeorge L Mendz
Apr 8, 2021·Critical Reviews in Microbiology·Kevin R TheisNardhy Gomez-Lopez
May 30, 2021·Trends in Endocrinology and Metabolism : TEM·Madeline E GrahamMarc J Tetel
Jul 20, 2021·Journal of Bacteriology·Omar Abdul-RahimAlan J Wolfe

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Methods Mentioned

BETA
lavage
genotyping
PCR
electrophoresis
biopsy

Software Mentioned

R
QIIME
Trimmomatic
vegan
Ribosomal Database Project ( RDP ) classifier
mothur
Canoco

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