Association between Three Mutations, F1565C, V1023G and S996P, in the Voltage-Sensitive Sodium Channel Gene and Knockdown Resistance in Aedes aegypti from Yogyakarta, Indonesia

Juli Rochmijati WuliandariNancy Endersby-Harshman


Mutations in the voltage-sensitive sodium channel gene (Vssc) have been identified in Aedes aegypti and some have been associated with pyrethroid insecticide resistance. Whether these mutations cause resistance, alone or in combination with other alleles, remains unclear, but must be understood if mutations are to become markers for resistance monitoring. We describe High Resolution Melt (HRM) genotyping assays for assessing mutations found in Ae. aegypti in Indonesia (F1565C, V1023G, S996P) and use them to test for associations with pyrethroid resistance in mosquitoes from Yogyakarta, a city where insecticide use is widespread. Such knowledge is important because Yogyakarta is a target area for releases of Wolbachia-infected mosquitoes with virus-blocking traits for dengue suppression. We identify three alleles across Yogyakarta putatively linked to resistance in previous research. By comparing resistant and susceptible mosquitoes from bioassays, we show that the 1023G allele is associated with resistance to type I and type II pyrethroids. In contrast, F1565C homozygotes were rare and there was only a weak association between individuals heterozygous for the mutation and resistance to a type I pyrethroid. As the heterozygote i...Continue Reading


Sep 16, 2000·Insect Biochemistry and Molecular Biology·J Hemingway
May 29, 2003·Insect Biochemistry and Molecular Biology·D M Soderlund, D C Knipple
Aug 5, 2010·The American Journal of Tropical Medicine and Hygiene·Angela F HarrisHilary Ranson
Jul 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yuzhe DuKe Dong
Jan 17, 2014·Parasites & Vectors·Jutta Gerlinde Birggitt LinssAdemir Jesus Martins


Jun 25, 2019·PloS One·Tri Baskoro Tunggul Satoto Kuswati
Apr 18, 2020·PLoS Neglected Tropical Diseases·Yinjun FanJeffrey G Scott
May 8, 2020·Archives of Insect Biochemistry and Physiology·Mengli ChenKe Dong
Jan 24, 2019·BioMed Research International·Penny Humaidah HamidCarlos Hermosilla
Aug 28, 2020·Veterinary World·Vika Ichsania NindityaPenny Humaidah Hamid
May 15, 2018·Journal of Vector Ecology : Journal of the Society for Vector Ecology·Nguyen Thi Kim LienNguyen Thi Huong Binh
Dec 19, 2017·PloS One·Penny Humaidah HamidCarlos Hermosilla
May 16, 2019·Veterinary World·Riesna Martianasari, Penny Humaidah Hamid