Association between tumoral GH-releasing peptide receptor type 1a mRNA expression and in vivo response to GH-releasing peptide-6 in ACTH-dependent Cushing's syndrome patients

European Journal of Endocrinology
Marcio Carlos MachadoL R Salgado

Abstract

GH secretagogues (GHS) produce exaggerated ACTH and cortisol responses in Cushing's disease (CD) patients, attributable to their direct action on GH-releasing peptide receptor type 1a (GHSR-1a). However, there are no studies correlating the in vivo response to GHS and GHSR-1a mRNA expression in ACTH-dependent Cushing's syndrome (CS) patients. The aim of this study is to correlate the patterns of ACTH and cortisol response to GH-releasing peptide-6 (GHRP-6) to GHSR-1a expression in ACTH-dependent CS patients. Prospective study in a tertiary referral hospital center. Fifteen CD patients and two ectopic ACTH syndrome (EAS) patients were studied. Tumor fragments were submitted to RNA extraction, and GHSR-1a expression was studied through real-time qPCR and compared with normal tissue samples. The patients were also submitted to desmopressin test and vasopressin receptor type 1B (AVPR1B) mRNA analysis by qPCR. GHSR-1a expression was similar in normal pituitary samples and in corticotrophic tumor samples. GHSR-1a expression was higher in patients (CD and EAS) presenting in vivo response to GHRP-6. Higher expression of AVPR1B was observed in the EAS patients responsive to desmopressin, as well as in corticotrophic tumors, as compared ...Continue Reading

Citations

Aug 28, 2010·International Journal of Peptides·Johannes D Veldhuis, Cyril Y Bowers
Sep 16, 2009·Current Opinion in Ophthalmology·Andrew M HendrickRibhi Hazin
Jun 19, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Stanley A Plotkin
Mar 31, 2015·Endocrine Journal·Yoshihiro SekiguchiYukio Hirata
Dec 4, 2020·International Journal of Molecular Sciences·Hidenori FukuokaYutaka Takahashi

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