Association of a bovine CXCR2 gene polymorphism with neutrophil survival and killing ability

Veterinary Immunology and Immunopathology
Magdalena RambeaudG M Pighetti

Abstract

Recent research in our lab has demonstrated a significant association between the incidence of subclinical mastitis and specific polymorphisms of the CXCR2 gene in Holstein dairy cows. This gene encodes a receptor for interleukin-8 (IL-8), a key regulator of neutrophil migration, killing and survival. Because of the importance of this gene in neutrophil function, we hypothesized that differences in neutrophil killing and survival may exist among the CXCR2 genotypes and potentially contribute to the observed variation in intramammary infections. To test this hypothesis, neutrophils were isolated from cows representing each CXCR2 +777 genotype (GG, GC or CC) and tested for suppression of apoptosis, reactive oxygen species (ROS) generation, glutathione levels, and bactericidal activity. A significant increase in survival was observed in neutrophils from cows with a CC genotype when compared to those with a GG genotype in response to IL-8, but not dexamethasone. In contrast, a significant reduction in neutrophil ROS generation in response to phorbol-13-myristate-12 acetate (PMA) was observed in cows with a CC genotype when compared to those with a GG genotype. However, no differences in bactericidal activity or glutathione levels w...Continue Reading

References

Mar 11, 1991·Veterinary Immunology and Immunopathology·M G StevensP C Canning
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·S A JonesM Baggiolini
Jan 5, 2000·Journal of Immunological Methods·S F van EedenJ C Hogg
May 10, 2000·American Journal of Veterinary Research·D McClenahanD Weiss
Aug 26, 2000·Advances in Experimental Medicine and Biology·M J PaapeC Burvenich
Sep 15, 2000·Journal of Immunological Methods·A K LehmannA Halstensen
Jan 4, 2001·Immunological Reviews·O ChertovJ J Oppenheim
Apr 5, 2001·Clinical Immunology : the Official Journal of the Clinical Immunology Society·N D Burg, M H Pillinger
Aug 10, 2004·Immunogenetics·S M YoungermanG M Pighetti
Aug 26, 2004·Journal of Dairy Science·S M YoungermanG M Pighetti
Jul 26, 2005·Infection and Immunity·M Rambeaud, G M Pighetti

❮ Previous
Next ❯

Citations

Mar 20, 2008·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Eveline M Ibeagha-AwemuXin Zhao
Oct 15, 2011·Journal of Mammary Gland Biology and Neoplasia·Gina M Pighetti, A A Elliott
Aug 11, 2010·The Journal of Veterinary Medical Science·Shuichi TsuchidaKazuhiko Kotani
Nov 8, 2011·Veterinary Immunology and Immunopathology·Gina M PighettiMagdalena Rambeaud
Nov 3, 2010·Veterinary Immunology and Immunopathology·Manuela RinaldiAnthony V Capuco
Oct 28, 2006·Veterinary Immunology and Immunopathology·Manuela RinaldiDouglas D Bannerman
Aug 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wendy W J van de SandeAlex van Belkum
Feb 16, 2018·Cell and Tissue Research·Laura L Bassel, Jeff L Caswell
Aug 6, 2017·Veterinary Immunology and Immunopathology·Lydia SiebertGina M Pighetti

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis