Association of a functional inducible nitric oxide synthase promoter variant with complications in type 2 diabetes

Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte
B J MorrisLi Wang

Abstract

Complications of diabetes have a genetic influence. Since increased inducible nitric oxide synthase (iNOS) gene ( NOS2A) expression can contribute to tissue damage, NOS2A is a worthy candidate for such a role. We therefore tested a 4-bp insertion/deletion (+/-) polymorphism 0.7 kb upstream of NOS2A for association with complications in type 2 diabetes patients, and also performed transient transfection experiments to examine the effect of this variant on promoter activity in kidney cells in culture. We investigated 379 Caucasian type 2 diabetes patients of British/European descent, 93 of whom had microalbuminuria, 26 overt nephropathy, 46 retinopathy, and 73 clinical neuropathy. Genotyping for the variant was carried out by PCR and automated Genescan analysis. Transient transfection studies involved the renal HEK 293 cell line and luciferase reporter gene constructs containing 1.1 kb of 5'-flanking DNA from '+' or '-' allele homozygotes. We found that the '+' allele frequency in patients without microalbuminuria was 12%, but was 23% in those with microalbuminuria ( P=0.0005), and was 26% in those with nephropathy ( P=0.0007), 22% in those with retinopathy ( P=0.037), and 23% in those with neuropathy ( P=0.045). The odds ratios ...Continue Reading

Citations

Mar 1, 2007·Indian Journal of Clinical Biochemistry : IJCB·Y S MahindrakarK M Kumbhar
Feb 25, 2003·Lancet·William Y S Wang, David J Adams
Apr 28, 2005·European Journal of Human Genetics : EJHG·Matthew J BugejaGraeme J Stewart
Dec 13, 2003·Biological Chemistry·Hartmut KleinertUlrich Förstermann
Apr 22, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Jörg MuntwylerMartin Hersberger
Feb 14, 2004·Redox Report : Communications in Free Radical Research·Jürgen F J Kun
May 5, 2010·Nitric Oxide : Biology and Chemistry·Andrea PautzHartmut Kleinert
Jul 29, 2009·Nitric Oxide : Biology and Chemistry·Jezabel VaradéElena Urcelay
Apr 28, 2009·Journal of Diabetes and Its Complications·Renata A BagarolliSara Teresinha O Saad
Nov 3, 2007·European Journal of Pharmacology·Dongzhe SongCatherine C Y Pang
Jun 6, 2007·Medical Hypotheses·Allam Appa RaoUndurti N Das
Oct 31, 2006·Journal of Diabetes and Its Complications·Lin LiaoKok-Onn Lee
Feb 11, 2011·Diabetes/metabolism Research and Reviews·Cristina GonzálezVisitación López-Miranda
Jun 21, 2012·IUBMB Life·Ka BianFerid Murad
Jan 27, 2012·Journal of Clinical Periodontology·Toru NishikawaTatsuaki Matsubara
Mar 25, 2010·Arthritis Research & Therapy·Rogelio J Palomino-MoralesJavier Martin
Aug 8, 2006·Journal of Pharmacological Sciences·Ka BianFerid Murad
May 12, 2010·Autoimmunity Reviews·Kim-Heang LyLuc Mouthon
Sep 19, 2006·Genetics in Medicine : Official Journal of the American College of Medical Genetics·John P A Ioannidis, Fotini K Kavvoura
Sep 30, 2010·American Journal of Physiology. Endocrinology and Metabolism·Phillip J WhiteAndré Marette
Aug 4, 2011·American Journal of Physiology. Endocrinology and Metabolism·Carolina Centeno-BaezAndré Marette
Apr 17, 2003·Current Opinion in Nephrology and Hypertension·M Luiza Caramori, Michael Mauer
Nov 26, 2003·European Journal of Clinical Investigation·T A KunnasP J Karhunen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.