Association of ABCC2 and CDDP-Resistance in Two Sublines Resistant to CDDP Derived from a Human Nasopharyngeal Carcinoma Cell Line.

Journal of Oncology
Si Ming XieXue Yun Zhong

Abstract

Cisplatin (CDDP) is one of the most active drugs to treat nasopharyngeal carcinoma (NPC) patients. To further understand the mechanisms of CDDP-resistance in NPC, two CDDP-resistant sublines (CNE2-CDDP and CNE2-CDDP-5Fu) derived from parental NPC cell line CNE2 were established. It was found that at the IC50 level, the resistance of CNE2-CDDP and CNE2-CDDP-5Fu against CDDP was 2.63-fold and 5.35-fold stronger than that of parental CNE2, respectively. Of the four ABC transporters (ABCB1, ABCC1, ABCC2 and ABCG2) related to MDR, only ABCC2 was found to be elevated both in CDDP-resistant sublines, with ABCC2 located in nucleus of CNE2-CDDP-5Fu but not in CNE2-CDDP and parental CNE2. Further research showed that compared to untreated CNE2, the intracellular levels of CDDP were decreased by 2.03-fold in CNE2-CDDP and 2.78-fold in CNE2-CDDP-5Fu. After treatment with PSC833, a modulator of MDR associated transporters including ABCC2, the intracellular level of CDDP was increased in CDDP-resistant sublines, and the resistance to CDDP was partially reversed from 2.63-fold to 1.62-fold in CNE2-CDDP and from 5.35-fold to 4.62-fold in CNE2-CDDP-5Fu. These data indicate that ABCC2 may play an important role in NPC resistant to CDDP.

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Citations

Nov 13, 2014·Metallomics : Integrated Biometal Science·Thiago de O AraujoReinaldo Calixto de Campos
Jan 29, 2011·Journal of Pharmaceutical Sciences·Sayo HoribeMikihisa Takano
Sep 19, 2012·Journal of Experimental & Clinical Cancer Research : CR·Adel M ZaatarChoong-Chin Liew
Jun 5, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Wentao LiWei You
Feb 10, 2018·Oncotarget·Lei ZhouXiao-Dong Zhu
Oct 30, 2020·Pharmacogenomics and Personalized Medicine·Nadiya Nurul AfifahMelisa I Barliana

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BETA
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PCR

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SPSS
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