Association of autoantibodies with Ku and DNA repair proteins in connective tissue diseases

Rheumatology
C Schild-PoulterR J G Haché

Abstract

To analyse the autoimmune response to DNA damage response factors in systemic autoimmune rheumatic disease (SARD) patients and to determine their association with autoantibodies to Ku antigen. We have screened the serum of 239 patients suffering from SARD, including systemic lupus erythematosus, systemic sclerosis and rheumatoid arthritis to detect the occurrence of autoantibodies to Ku and four other DNA damage response factors that form macromolecular complexes with Ku using an immunoprecipitation assay. We identified samples positive for autoantibodies to Ku (20.5%), DNA-dependent protein kinase catalytic subunit (DNA-PKcs, 8.4%) and poly(ADP-ribose) polymerase (5.9%), and report for the first time autoantibodies directed against two additional DNA repair proteins, Werner (6.3%) and Mre11 (9.6%). Remarkably, we found a striking correlation between the production of antibodies to Ku and the other four Ku-binding factors. Sixty-five percent of anti-Ku-positive sera were found to contain at least one of the four anti-DNA repair antibodies vs only 10% of the anti-Ku-negative sera. Our results suggest that the autoantibodies directed against Ku are elicited by macromolecular protein complexes containing Ku and the associated DNA ...Continue Reading

Citations

Jun 30, 2012·Autoimmunity Reviews·Sonal MehraMarvin J Fritzler
Aug 25, 2016·Frontiers in Genetics·Rania Nakad, Björn Schumacher
Jul 14, 2010·Autoimmunity Reviews·C BeliznaH Lévesque
Feb 7, 2017·Neurochemistry International·Noriko Yasuhara, Yoshihiro Yoneda
Sep 2, 2016·Medicine·S HoaUNKNOWN Canadian Scleroderma Research Group (CSRG); Australian Scleroderma Interest Group (ASIG); Genetics versus Environment in Scl
Aug 18, 2018·Journal of Immunology Research·Martha Paola Mireles-CanalesCésar Pacheco-Tena
Oct 31, 2020·Frontiers in Immunology·Nikolaos I VlachogiannisPetros P Sfikakis
Jul 28, 2019·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Qingxia ShenJae Hoon Lee
Jan 27, 2021·Annals of the Rheumatic Diseases·Brittany L AdlerAntony Rosen
Apr 16, 2021·Cellular and Molecular Life Sciences : CMLS·Sanna AbbasiCaroline Schild-Poulter
Jun 3, 2021·International Journal of Molecular Sciences·Theodora ManolakouDimitrios T Boumpas
Jun 19, 2021·Seminars in Arthritis and Rheumatism·Maria Casal-DominguezAndrew L Mammen
Aug 13, 2021·Frontiers in Immunology·Benjamin Klein, Claudia Günther

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Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

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