Association of branched oligonucleotides into the i-motif

Journal of Biomolecular Structure & Dynamics
S RobidouxM J Damha

Abstract

The unique architecture of branched oligonucleotides mimicking lariat RNA introns [Wallace and Edmons, Proc. Natl. Acad. Sci. USA 80, 950-954 (1983)] was exploited to study compounds that associate as two parallel duplexes with intercalating C/C+ base pairs (i-motif DNA) [Gehring et al. Nature 363, 561-565 (1993)]. The formation of a branched cytosine tetrad was induced by joining the 5'-ends of pair of pentadeoxycytidine strands with a branching riboadenosine (rA) linker. This arrangement causes the orientation of the dC strands to be parallel, and forces the formation of a C/C+ duplex that self-associates into i-DNA. Presence of the i-motif in this structure is supported by thermal denaturation, native gel electrophoresis, CD, and NMR spectroscopy.

References

Jul 29, 1988·Science·J H van de SandeT M Jovin
Jan 1, 1984·Annual Review of Biochemistry·A RichA H Wang
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·C KangA Rich
Nov 22, 1994·Biochemistry·L ChenA Rich
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·C H KangA Rich
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·I BergerA Rich
May 30, 1953·Nature·J D WATSON, F H CRICK
May 25, 1963·Nature·R LANGRIDGE, A RICH
Mar 1, 1963·Biochemistry·E O AKINRIMISIP O TS'O
Jan 1, 1995·Journal of the American Chemical Society·Squire Rumney, Eric T Kool
Oct 1, 1995·Journal of the American Chemical Society·Narayan C Chaudhuri, Eric T Kool

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Citations

Jun 14, 2000·Current Opinion in Structural Biology·M Guéron, J L Leroy
Jan 24, 1998·Journal of Biomolecular Structure & Dynamics·S Robidoux, M J Damha
Apr 23, 2008·Current Protocols in Nucleic Acid Chemistry·Sandra Carriero, Masad J Damha
Nov 8, 2001·Journal of Biomolecular Structure & Dynamics·S S PataskarS K Brahmachari
Nov 3, 2017·Chemistry : a European Journal·Hala Abou AssiMasad J Damha
Aug 21, 2018·Nucleic Acids Research·Hala Abou AssiMasad J Damha

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