PMID: 3755057Jun 17, 1986Paper

Association of calmodulin with peptide analogues of the inhibitory region of the heat-stable protein inhibitor of adenosine cyclic 3',5'-phosphate dependent protein kinase

Biochemistry
D A MalencikS R Anderson

Abstract

A 20-residue peptide analogue (IASGRTGRRNAIHDILVSSA) of the 8000-dalton heat-stable cAMP-dependent protein kinase inhibitor undergoes efficient calcium-dependent binding by calmodulin, with Kd approximately 70 nM when calcium is present. It is a potent inhibitor of smooth muscle myosin light chain kinase and of the calmodulin-dependent phosphatase activity of calcineurin. At concentrations above 3 microM, the peptide stimulates the basal activity of calcineurin. The native protein kinase inhibitor has no effect on the catalytic activity of myosin light chain kinase and is moderately inhibitory to both the calmodulin-dependent and -independent phosphatase activity of calcineurin. Competition experiments using excess concentrations of calcineurin and calmodulin suggest that the primary interaction of the native heat-stable inhibitor is with the catalytic subunit of protein kinase. Dansylcalmodulin exhibits only a weak interaction with the inhibitor. Observations on deletion peptides of the 20-residue analogue help to delineate the overlapping peptide binding specificities of the cAMP-dependent protein kinase [Scott, J. D., Glaccum, M. B., Fischer, E. H., & Krebs, E. G. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 1613-1616] and calmo...Continue Reading

References

Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·C O BrostromD J Wolff
Oct 6, 1975·Biochemical and Biophysical Research Communications·W Y CheungE A Tallant
Jan 1, 1978·Annual Review of Biochemistry·P Y Chou, G D Fasman
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J D ScottE G Krebs
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J D ScottE G Krebs
Feb 11, 1986·Biochemistry·D A Malencik, S R Anderson
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D K BlumenthalE G Krebs
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A J Bendich, R S Anderson
May 22, 1984·Biochemistry·D A Malencik, S R Anderson
Jul 6, 1982·Biochemistry·D A Malencik, S R Anderson
Apr 12, 1983·Biochemistry·D A Malencik, S R Anderson
Jan 1, 1980·Annals of the New York Academy of Sciences·H Rasmussen
May 4, 1983·Biochimica Et Biophysica Acta·C Villar-PalasiR H Kretsinger
Jan 23, 1984·FEBS Letters·H HinssenA Sobieszek
Jan 1, 1984·Advances in Enzyme Regulation·J E Casnellie, E G Krebs
Sep 16, 1982·Biochemical and Biophysical Research Communications·D A MalencikS R Anderson
Jul 30, 1981·Biochemical and Biophysical Research Communications·D A MalencikM I Schimerlik

❮ Previous
Next ❯

Citations

Feb 1, 1988·Journal of Protein Chemistry·E S LovegrenD Puett
Feb 9, 1988·Biochemistry·D A Malencik, S R Anderson
Mar 22, 1988·Biochemistry·D A Malencik, S R Anderson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.