Association of (c)AMP-degrading glycosylphosphatidylinositol-anchored proteins with lipid droplets is induced by palmitate, H2O2 and the sulfonylurea drug, glimepiride, in rat adipocytes

Biochemistry
G MüllerW Frick

Abstract

Inhibition of lipolysis in rat adipocytes by palmitate, H2O2 and the antidiabetic sulfonylurea drug, glimepiride, has been demonstrated to rely on the upregulated conversion of cAMP to adenosine by enzymes associated with lipid droplets (LD) rather than on cAMP degradation by the insulin-stimulated microsomal phosphodiesterase 3B (Müller, G., Wied, S., Over, S., and Frick, W. (2008) Biochemistry 47, 1259-1273). Here these two enzymes were identified as the glycosylphosphatidylinositol (GPI)-anchored phosphodiesterase, Gce1, and the 5'-nucleotidase, CD73, on basis of the following findings: (i) Photoaffinity labeling with 8-N3-[32P]cAMP and [14C]5'-FSBA of LD from palmitate-, glucose oxidase- and glimepiride-treated, but not insulin-treated and basal, adipocytes led to the identification of 54-kDA cAMP- and 62-kDa AMP-binding proteins. (ii) The amphiphilic proteins were converted into hydrophilic versions and released from the LD by chemical or enzymic treatments specifically cleaving GPI anchors, but resistant toward carbonate extraction. (iii) The cAMP-to-adenosine conversion activity was depleted from the LD by adsorption to (c)AMP-Sepharose. (iv) cAMP-binding to LD was increased upon challenge of the adipocytes with palmitat...Continue Reading

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Citations

Aug 27, 2009·Journal of Cell Science·Neville CobbeMargarete M S Heck
Aug 28, 2012·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Günter Müller
Jan 8, 2010·Archives of Physiology and Biochemistry·Günter MüllerGabriele Biemer-Daub
Feb 20, 2010·Archives of Physiology and Biochemistry·Günter MüllerWendelin Frick
Aug 9, 2011·Life Sciences·Tomasz Szkudelski, Katarzyna Szkudelska
Sep 14, 2016·Biological Chemistry·Ching-Wen ChangMargarete M S Heck
Aug 15, 2013·Bioinformatics and Biology Insights·Sonia J MoisáJuan J Loor
Dec 7, 2010·Metabolism: Clinical and Experimental·Günter MüllerGabriele Biemer-Daub

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