Association of circulating hepatitis G virus with lipoproteins for a lack of binding with antibodies

Biochemical and Biophysical Research Communications
K SatoM Mayumi

Abstract

Buoyant density of a recently discovered putative non-A to E hepatitis virus designated hepatitis G virus (HGV) was estimated in plasma or serum samples from three symptom-free carriers and two hepatitis patients. HGV RNA was detected by reverse-transcription polymerase chain reaction in sucrose density fractions with a low density at 1.05-1.10 g/cm3, and the density shifted to 1.23-1.26 g/cm3 after a treatment of peak fractions with Tween 80. Fractionated HGV was precipitated with antibodies to apolipoproteins, but not at all with antibodies to IgG; it was retained by affinity columns of lectins. These results indicate that the circulating HGV would be covered with lipoproteins of the host and has sugar moieties on the surface. The association of HGV with lipids would be responsible for the observed low density and prevent the binding with antibodies for viral persistence.

Citations

Apr 12, 2000·Journal of Viral Hepatitis·J XiangJ T Stapleton
Nov 4, 2000·Journal of Gastroenterology and Hepatology·T KandaH Saisho
Mar 13, 1997·The New England Journal of Medicine·Y Miyakawa, M Mayumi
Feb 23, 2010·Journal of Lipid Research·Juan GuevaraNatalia Valentinova Guevara
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·V AgnelloQ X Zhang
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Feb 12, 1998·Biochemical and Biophysical Research Communications·T NishizawaM Mayumi

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