Association of Ct Values from Real-Time PCR with Culture in Microbiological Clearance Samples for Shiga Toxin-Producing Escherichia coli (STEC)

Microorganisms
Michael Bording-JorgensenLinda Chui

Abstract

Shiga toxin-producing Escherichia coli (STEC) are associated with acute gastroenteritis worldwide, which induces a high economic burden on both healthcare and individuals. Culture-independent diagnostic tests (CIDT) in frontline microbiology laboratories have been implemented in Alberta since 2019. The objectives of this study were to determine the association between gene detection and culture positivity over time using STEC microbiological clearance samples and also to establish the frequency of specimen submission. Both stx genes' amplification by real-time PCR was performed with DNA extracted from stool samples using the easyMAG system. Stools were inoculated onto chromogenic agar for culture. An association between gene detection and culture positivity was found to be independent of which stx gene was present. CIDT can provide rapid reporting with less hands-on time and technical expertise. However, culture is still important for surveillance and early cluster detection. In addition, stool submissions could be reduced from daily to every 3-5 days until a sample is negative by culture.

References

Nov 1, 2001·Research in Microbiology·H Schmidt
Mar 18, 2006·Journal of Food Protection·S E MajowiczJ B Wilson
Jul 25, 2008·International Journal of Food Microbiology·S J HensonA Q Jones
Oct 2, 2008·Emerging Infectious Diseases·Robert L AtmarDavid Y Graham
Jan 29, 2011·Journal of Biomedicine & Biotechnology·Krystle L Mohawk, Alison D O'Brien
Apr 11, 2012·International Journal of Molecular Sciences·Daniel D RhoadsScot E Dowd
Jan 10, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ralf P VonbergSebastian Suerbaum
Aug 1, 2010·Journal of Statistical Software·Michael P Fay, Pamela A Shaw
May 26, 2015·Clinics in Laboratory Medicine·Allen BryanAlexander J McAdam
May 10, 2016·Diagnostic Microbiology and Infectious Disease·Nathan ZelyasLinda Chui
Oct 22, 2017·Médecine et maladies infectieuses·M BruyandC Loirat
Feb 8, 2018·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·M Kate ThomasFrank Pollari
May 18, 2018·Canada Communicable Disease Report = Relevé Des Maladies Transmissibles Au Canada·V MortonA Kearney
Jul 5, 2019·Journal of Clinical Microbiology·William StokesDylan R Pillai
Nov 30, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Laura Fernanda AlconcherMabel Rizzo

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BETA
PCR

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