Association of dopamine D(3) receptors with actin-binding protein 280 (ABP-280)

Biochemical Pharmacology
Ming LiQun Yong Zhou

Abstract

Proteins that bind to G protein-coupled receptors have been identified as regulators of receptor localization and signaling. In our previous studies, a cytoskeletal protein, actin-binding protein 280 (ABP-280), was found to associate with the third cytoplasmic loop of dopamine D(2) receptors. In this study, we demonstrate that ABP-280 also interacts with dopamine D(3) receptors, but not with D(4) receptors. Similar to the dopamine D(2) receptor, the D(3)/ABP-280 association is of signaling importance. In human melanoma M2 cells lacking ABP-280, D(3) receptors were unable to inhibit forskolin-stimulated cyclic AMP (cAMP) production significantly. D(4) receptors, however, exhibited a similar degree of inhibition of forskolin-stimulated cAMP production in ABP-280-deficient M2 cells and ABP-280-replent M2 subclones (A7 cells). Further experiments revealed that the D(3)/ABP-280 interaction was critically dependent upon a 36 amino acid carboxyl domain of the D(3) receptor third loop, which is conserved in the D(2) receptor but not in the D(4) receptor. Our results demonstrate a subtype-specific regulation of dopamine D(2)-family receptor signaling by the cytoskeletal protein ABP-280.

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Citations

Nov 6, 2010·Archives of Pharmacal Research·Dong Im ChoKyeong-Man Kim
Nov 19, 2005·Blood·Fumihiko NakamuraJari Ylänne
Sep 3, 2010·PloS One·Laura MinsaasAnna M Aragay
Sep 22, 2010·Neurochemical Research·Eric J Simon, Irma Onoprishvili
Apr 20, 2004·The European Journal of Neuroscience·Magali PeetersEmmanuel Hermans
Nov 1, 2006·Biochimica Et Biophysica Acta·Chunmin DongGuangyu Wu
Nov 4, 2004·Journal of Receptor and Signal Transduction Research·Kim A NeveHeather Trantham-Davidson

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