Association of Drosophila cysteine string proteins with membranes

FEBS Letters
J van de Goor, R Kelly

Abstract

Cysteine string proteins are putative synaptic vesicle proteins that lack a transmembrane domain. Our analysis shows that Drosophila cysteine string proteins are extensively modified by hydroxylamine-sensitive fatty acylation. This modification could be responsible for association of csp's with membranes. Extensive deacylation of Dcsp's by a 20 h incubation in 1 M hydroxylamine, pH 7.0, or methanolic KOH produces a protein of 6-7 kDa lower mass than untreated Dcsp's. Surprisingly, the hydroxylamine treatment does not cause release of Dcsp's from membranes. On the other hand, alkaline stripping of membranes isolated from Drosophila brain by 0.1 M sodium carbonate, pH 11.5, causes a significant release of Dcsp's from membranes into the cytosol. These results indicate that fatty acylation may not form the main anchor of Dcsp's in membranes. Taking advantage of the endocytotic block in the Drosophila mutant shibire ts1, we analyzed the acylation states of Dcsp's in two stages during synaptic vesicle recycling and found no evidence for an acylation/deacylation cycle of Dcsp's in the brain nerve terminals.

References

Nov 1, 1990·Journal of Neurogenetics·K E ZinsmaierE Buchner
Apr 1, 1990·The Journal of Cell Biology·N PfannerJ E Rothman
Apr 10, 1984·Biochimica Et Biophysica Acta·A I MageeM J Schlesinger
Jan 1, 1995·Brain Research. Molecular Brain Research·A Mastrogiacomo, C B Gundersen
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·J van de GoorR Kelly
Jul 1, 1994·Cell·P B Wedegaertner, H R Bourne
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·M G EasonS B Liggett
Mar 1, 1994·Journal of Neurochemistry·A MastrogiacomoC B Gundersen
Dec 3, 1993·Cell·J OstermannJ E Rothman

❮ Previous
Next ❯

Citations

May 1, 1997·Trends in Neurosciences·E Buchner, C B Gundersen
Jul 4, 2008·The Journal of Biological Chemistry·Jennifer GreavesLuke H Chamberlain
Apr 4, 1998·Molecular Biology of the Cell·S Gonzalo, M E Linder
Aug 7, 1998·Molecular Biology of the Cell·L H Chamberlain, R D Burgoyne
Nov 1, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Chun-Fai NgClara Bik-San Lau
Jun 11, 2009·Journal of Neurochemistry·Gerald R PrescottLuke H Chamberlain
Mar 25, 1999·Cell·T CasciM Freeman
Mar 25, 2015·Seminars in Cell & Developmental Biology·Robert D Burgoyne, Alan Morgan
Oct 15, 1998·FEBS Letters·A MastrogiacomoC B Gundersen
Nov 23, 2006·Biochemical and Biophysical Research Communications·Liping BaiJanice E A Braun
May 9, 2000·Journal of Neurochemistry·L H Chamberlain, R D Burgoyne
Jun 30, 2010·Journal of Neurogenetics·Konrad E Zinsmaier
Feb 12, 2020·Progress in Neurobiology·Cameron B Gundersen
Mar 11, 2004·The Journal of Experimental Biology·Christine ArnoldErich Buchner
May 30, 2001·Biochemical Pharmacology·K E Zinsmaier, P Bronk

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.