Association of electrospinning with electrospraying: a strategy to produce 3D scaffolds with incorporated stem cells for use in tissue engineering

International Journal of Nanomedicine
Daikelly Iglesias BraghirolliPatricia Pranke

Abstract

In tissue engineering, a uniform cell occupation of scaffolds is crucial to ensure the success of tissue regeneration. However, this point remains an unsolved problem in 3D scaffolds. In this study, a direct method to integrate cells into fiber scaffolds was investigated by combining the methods of electrospinning of fibers and bioelectrospraying of cells. With the associating of these methods, the cells were incorporated into the 3D scaffolds while the fibers were being produced. The scaffolds containing cells (SCCs) were produced using 20% poly(lactide-co-glycolide) solution for electrospinning and mesenchymal stem cells from deciduous teeth as a suspension for bioelectrospraying. After their production, the SCCs were cultivated for 15 days at 37°C with an atmosphere of 5% CO2. The 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide test demonstrated that the cells remained viable and were able to grow between the fibers. Scanning electron microscopy showed the presence of a high number of cells in the structure of the scaffolds and confocal images demonstrated that the cells were able to adapt and spread between the fibers. Histological analysis of the SCCs after 1 day of cultivation showed that the cells were unif...Continue Reading

Citations

Apr 6, 2016·Archives of Oral Biology·Stefanie Bressan WerleLuciano Casagrande
Apr 14, 2016·Nanomedicine·Manus BiggsDimitrios I Zeugolis
Aug 15, 2019·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D I BraghirolliP Pranke
Feb 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Luminita Nicoleta DumitrescuMaria Dinescu
Jan 1, 2016·Nanomaterials·Bogdanel Silvestru MunteanuCornelia Vasile
Aug 13, 2020·F1000Research·Citlali Helenes GonzálezPatrizia Ferretti
Jun 27, 2020·Biomedical Materials·Juliana Girón BastidasPatricia Pranke

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Methods Mentioned

BETA
scanning electron microscopy
confocal microscopy

Software Mentioned

ImageJ
Qcapture

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