Association of equine sperm population parameters with outcome of intracytoplasmic sperm injections

Theriogenology
Raul A Gonzalez-Castro, Elaine M Carnevale

Abstract

Limited clinical information is available regarding sperm population parameters that are important for use with equine intracytoplasmic sperm injection (ICSI). Therefore, the appropriateness of a sample of sperm is typically not known before ICSI. The aim of our study was to determine which sperm population characteristics were predictive of ICSI outcome. Frozen-thawed sperm samples (n = 114) from 37 stallions in a clinical program were analyzed after ICSI for percentages of normal morphology (MORPH+), live as assessed by eosin/nigrosin stain (LIVE+), membrane intact as assessed by hypoosmotic swelling test (HOS+), and DNA fragmentation determined by sperm chromatin dispersion (DNA-). ICSI was performed on 147 oocytes, and cleavage (≥2 cells), embryo development (morula or blastocyst), and pregnancy status after embryo transfer were determined. Among the examined sperm parameters, LIVE + correlated positively with MORPH+ and HOS+, and MORPH + negatively with DNA-; no other significant correlations were observed. When used for ICSI, sperm population percentages for MORPH+ and DNA- were not predictive of ICSI outcome, including cleavage, embryo development, and establishment of a pregnancy. Sperm population percentages significan...Continue Reading

References

Jan 1, 1996·Annual Review of Physiology·L S King, P Agre
Sep 1, 1996·Journal of Reproduction and Fertility·C G GravanceP J Casey
Mar 23, 2000·Theriogenology·D NeildA Agüero
Dec 29, 2000·Andrologia·D M NeildA Agüero
Apr 13, 2005·Theriogenology·A H WaltersF C Gwazdauskas
Oct 11, 2005·Fertility and Sterility·José Luis FernándezChristopher De Jonge
Jun 3, 2006·Reproductive Biomedicine Online·Donald Evenson, Regina Wixon
Oct 5, 2006·Journal of Assisted Reproduction and Genetics·Zhongxiang LiHefeng Huang
Jul 24, 2007·Fertility and Sterility·John A CollinsPeter N Schlegel
Sep 8, 2007·International Journal of Andrology·Hassan W BakosMichelle Lane
Jan 9, 2008·Acta Veterinaria Scandinavica·Jane M MorrellHeriberto Rodriguez-Martinez
Apr 29, 2008·Fertility and Sterility·Conrado AvendañoSergio Oehninger
Jul 12, 2008·Reproductive Biomedicine Online·J G FrancoL Vagnini
Nov 6, 2008·Reproductive Biomedicine Online·Andrea GarollaCarlo Foresta
Jun 10, 2009·Fertility and Sterility·Myung-Geol PangYoung-Ju Kim
Aug 1, 2009·Urology·Sergey I MoskovtsevJ Brendan M Mullen
Sep 14, 2010·Reproductive Biomedicine Online·James D StangerGhanim Almahbobi
Jan 5, 2011·Theriogenology·Sabine HeckenbichlerChristine Aurich
May 19, 2012·Human Reproduction·F BassiriM H Nasr-Esfahani
Jul 19, 2012·International Journal of Andrology·M H Nasr-EsfahaniM Tavalaee

❮ Previous
Next ❯

Citations

Oct 24, 2019·Reproduction, Fertility, and Development·Lee H Morris, Lisa J Maclellan
Oct 3, 2019·Reproduction, Fertility, and Development·Lino Fernando Campos-ChillonJoy L Altermatt
Jan 7, 2021·Animals : an Open Access Journal From MDPI·Brenda Florencia GimenoClaudia Elena Osycka-Salut

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians
Andrea L TranquilliStefano Giannubilo
The Australian & New Zealand Journal of Obstetrics & Gynaecology
Andrew KanMichael Chapman
© 2022 Meta ULC. All rights reserved