Association of functional influenza viral proteins and RNAs with nuclear chromatin and sub-chromatin structure

Microbes and Infection
Naoki TakizawaKyosuke Nagata

Abstract

Transcription and replication of the influenza virus genome occur in the nucleus. However, the intra-nuclear localization of viral RNP complexes and the function of nuclear domains involved in viral transcription and replication, if any, are not well known. In the present study, we determined the intra-nuclear localization of viral proteins and viral RNAs and the in vitro RNA synthesis activity of viral RNP complexes associated with distinct nuclear fractions prepared from infected nuclei. A majority of viral RNA polymerases and M1 were recovered in DNase-sensitive fractions, whereas some portion of RNA polymerases and approximately 25% of NP were tightly associated with so-called nuclear matrix fractions. The amount of vRNA associated with the nuclear matrix was significantly more than that of cRNA. The in vitro viral RNA synthesis activity was detected in DNase-insensitive fractions, including the nuclear matrix. In contrast, newly synthesized viral RNAs were recovered in the DNase-sensitive fraction. These observations suggest that vRNP complexes are, at least partially, associated with densely packed chromatin, where viral transcription and replication occur, and the newly synthesized vRNP complexes to be transported into t...Continue Reading

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Citations

Jun 15, 2012·Journal of Virology·Dorothée MoisyJean-Luc Jestin
Dec 30, 2014·Virology·Michiko KumakuraKyosuke Nagata
Oct 18, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·L JossetM Rosa-Calatrava
Apr 3, 2008·Reviews in Medical Virology·Kyosuke NagataTadasuke Naito
Dec 12, 2012·Virology·David M KnipeThomas M Kristie
Jul 12, 2016·Nature Reviews. Microbiology·Aartjan J W Te Velthuis, Ervin Fodor
Jan 23, 2016·Journal of Virology·Laura Marcos-VillarAmelia Nieto
Aug 26, 2006·Reviews in Medical Virology·Othmar G Engelhardt, Ervin Fodor
Mar 3, 2018·Archives of Virology·Naoki TakizawaMasakatsu Shibasaki
Jun 12, 2010·Expert Review of Proteomics·Yuki Furuse, Hitoshi Oshitani
Jul 1, 2020·Proceedings of the National Academy of Sciences of the United States of America·André SchreiberStephan Ludwig
May 14, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Young Hyun ParkJae Yong Han
Mar 27, 2021·Annual Review of Biochemistry·Tim KrischunsStephen Cusack

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