Association of GHR Polymorphisms with Milk Production in Buffaloes

Animals : an Open Access Journal From MDPI
S M El-KomyM A El-Magd

Abstract

For its role in the mediation of growth hormone (GH) galactopoietic effect, growth hormone receptor (GHR) was considered a functional candidate gene for milk performance in cattle. However, its genetic variation and potential effect have not been investigated in Egyptian buffaloes. This study aimed to screen GHR for polymorphisms and study their associations with milk traits in Egyptian buffaloes. Polymerase chain reaction, single-strand conformation polymorphism, and sequencing were used to identify mutations in 4 exons (E4-E6 and E8) of the GHR gene in 400 Egyptian buffaloes. No polymorphisms were found in E4, while 2 SNPs (c.380G>A/p.Arg127Lys and c.387C>T/p.Gly129) in E5, one silent mutation (c.435A>G/p.Pro145) in E6, and another missense mutation (c.836T>A/p.Phe279Tyr) in E8 were detected. The c.380G>A SNP in the extracellular domain was associated with milk yield, fat %, protein %, and 305-day milk, fat and protein yield, with higher levels in animals carrying the mutant A allele. The c.836T>A SNP in the transmembrane domain was associated with milk yield, fat %, protein %, and 305-day milk, fat and protein yield, with higher milk yield and lower fat %, protein %, fat and protein yield in the mutant A allele-animals. Inte...Continue Reading

References

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Citations

Sep 12, 2020·Domestic Animal Endocrinology·S M El-KomyM A El-Magd
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Saif Ur RehmanQingyou Liu
May 1, 2021·Animals : an Open Access Journal From MDPI·Mohammed A El-MagdShymaa M El-Komy

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Datasets Mentioned

BETA
KC107765

Methods Mentioned

BETA
PCR
Genotyping

Software Mentioned

primer
Image J
SAS
Geneious
StepOnePlus
GraphPad
PopGene
GenCal
PHASE
Haploview

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