Abstract
To clarify the association of glypican-3 (GPC3) expression with Wnt and other growth signaling molecules in hepatocellular carcinoma (HCC). Expression of GPC3, Wnt, matrix metalloproteinases (MMPs), sulfatase (SULF)1, SULF2, and other growth signaling molecules was analyzed in HCC cell lines and tissue samples by real-time reverse transcription-polymerase chain reaction, immunoblotting, and/or immunostaining. Expression of various genes in GPC3 siRNA-transfected HCC cells was analyzed. GPC3 was overexpressed in most HCCs at mRNA and protein levels and its serum levels were significantly higher in patients with HCC than in non-HCC subjects (P < 0.05). Altered expressions of various MMPs and growth signaling molecules, some of which were correlated with GPC3 expression, were observed in HCCs. Down-regulation of GPC3 expression by siRNA in GPC3-overexpressing HCC cell lines resulted in a significant decrease in expressions of MMP2, MMP14, fibroblast growth factor receptor 1, insulin-like growth factor 1 receptor. GPC3 expression was significantly correlated with nuclear/cytoplasmic localization of beta-catenin. These results suggest that GPC3, in conjunction with MMPs and growth signaling molecules, might play an important role in...Continue Reading
References
Mar 21, 1997·The Journal of Biological Chemistry·H H SongJ Filmus
Apr 1, 1997·Gastroenterology·H YamamotoK Imai
Sep 12, 2000·The American Journal of Pathology·H C HsuS Y Peng
Feb 15, 2001·Gut·H KanetoK Imai
Mar 15, 2001·Gut·Z W ZhuM W Büchler
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·X R XuZ G Han
Dec 13, 2002·International Journal of Cancer. Journal International Du Cancer·Yutaka MidorikawaHiroyuki Aburatani
Jun 5, 2003·Biochimica Et Biophysica Acta·Rachel H GilesHans Clevers
Jun 6, 2003·Biochemical and Biophysical Research Communications·Tetsuya NakatsuraYasuharu Nishimura
Jun 26, 2003·Cancer Science·Young Kwan SungMoonkyu Kim
Jul 10, 2003·Gastroenterology·Mariana CapurroJorge Filmus
Aug 12, 2003·Breast Cancer Research and Treatment·M G PetersE Bal de Kier Joffé
Dec 12, 2003·Lancet·Josep M LlovetJordi Bruix
Apr 3, 2004·Cancer Research·Yoshitaka HippoHiroyuki Aburatani
May 28, 2005·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Naoko YamauchiMasashi Fukayama
Jul 19, 2005·Cancer Research·Mariana I CapurroJorge Filmus
Feb 3, 2006·Cancer Research·Xinqiang HuangWallace L McKeehan
Jun 5, 2007·Oncogene·K KitisinL Mishra
Jun 16, 2007·Gastroenterology·Hashem B El-Serag, K Lenhard Rudolph
Aug 29, 2007·The Journal of Pathology·H TaniguchiY Shinomura
Jan 25, 2008·Journal of Biochemistry·Yoshie KoyamaKayoko Oguri
Mar 6, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jin-Ping LaiLewis R Roberts
Apr 12, 2008·Breast Cancer Research and Treatment·Ivan StiglianoMaría Giselle Peters
Apr 17, 2008·Carcinogenesis·Wei ChengYu-May Lee
Jun 21, 2008·Molecular Cancer Therapeutics·Wenhua PiaoKohzoh Imai
Jul 2, 2008·Journal of Gastroenterology·Hideyasu TakagiYasuhisa Shinomura
Oct 7, 2008·World Journal of Gastroenterology : WJG·Mariko OkiYasuhisa Shinomura
Nov 4, 2008·Archives of Pathology & Laboratory Medicine·Hanlin L WangXiming J Yang
Dec 3, 2008·Cancer Research·Takahiro IshiguroHisafumi Yamada-Okabe
Citations
May 11, 2011·Medical Oncology·Qiang LinMing Chen
Jan 7, 2016·Biological Chemistry·Mohammed M H Al-GayyarAhmed M Hamdan
Dec 2, 2015·Molecular and Clinical Oncology·Jian ZhangChaoyong Tu
Oct 20, 2012·Human Pathology·Taofic MounajjedTsung-Teh Wu
Aug 1, 2012·Seminars in Oncology·Gaetano BertinoGiuseppe Stefano Calvagno
Oct 13, 2012·Journal of Cellular Biochemistry·Hui-Lai MiaoMing Chen
Apr 24, 2013·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Hui-Lai MiaoMing-Yi Li
Dec 3, 2014·Archives of Medical Research·Xiaobo JiaZhi Du
May 7, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Min ChenHong Tu
Sep 4, 2015·PloS One·Wei GaoMitchell Ho
Jun 18, 2017·Medicinal Research Reviews·Fubo ZhouJie Tian
Nov 7, 2017·Biomaterials Science·Weitong SunXintao Shuai
Dec 25, 2017·Journal of Cell Communication and Signaling·Salma Abdi MahmoudJianwu Tang
May 6, 2020·The Journal of Clinical Investigation·Jennifer LaskowskiJoshua M Thurman
Jan 23, 2013·Molecular Medicine Reports·Zhijian PanFeixiang Chen
Nov 20, 2016·Macromolecular Bioscience·Zainen QinLei Zhu
Jan 31, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Andrew X ZhuPhilip A Philip
Dec 6, 2014·Molecular Cancer Research : MCR·Carolina M VicenteLeny Toma
Aug 8, 2021·Cancers·Isabel Theresa Schobert, Lynn Jeanette Savic
Nov 4, 2021·Investigative Ophthalmology & Visual Science·Tayler F L Wishart, Frank J Lovicu