Association of granulocyte transmigration with structural and cellular parameters of injury in experimental radiation enteropathy

Radiation Oncology Investigations
K K RichterM Hauer-Jensen

Abstract

Inflammatory cells are involved in the pathogenesis of tissue injury through release of cytokines and biologically active compounds. This study used a novel, noninvasive method to assess the association between granulocyte transmigration and structural and molecular changes in radiation enteropathy. A 4 cm loop of rat small intestine was exposed to 0, 2.8, 12, or 23 Gy localized irradiation. Feces was collected in metabolic cages before and 3, 7, 14, 28, and 42 days after irradiation. Granulocyte marker protein (GMP) was measured in buffer extracts of feces by enzyme-linked immunosorbent assay (ELISA). Irradiated and shielded intestine were procured at 2 and 26 weeks and assessed for histopathologic injury [radiation injury score (RIS)], ED-2 positive macrophages, and interleukin-1 alpha (IL-1 alpha) positive cells. Irradiated intestine exhibited characteristic histopathologic alterations and increased numbers of macrophages and IL-1 alpha positive cells. There was a highly significant dose-dependent increase in post-radiation GMP (P < 0.0001). Maximal GMP excretion occurred 3-7 days after irradiation. Six weeks after irradiation, GMP excretion had returned to normal in the 2.8 and 12 Gy groups, but was still 3.5 times higher i...Continue Reading

References

Sep 1, 1992·Scandinavian Journal of Gastroenterology·A G RøsethH Schjønsby
Jun 1, 1988·International Journal of Radiation Oncology, Biology, Physics·M Hauer-JensenJ W Osborne
Jan 1, 1983·Acta Radiologica. Oncology·M H JensenK Nygaard
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Jul 1, 1994·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·C W LangbergC J Kane
Dec 1, 1993·Scandinavian Journal of Gastroenterology·A G RøsethB Roald
Jun 1, 1996·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·K K RichterM Hauer-Jensen
Jul 1, 1996·Scandinavian Journal of Gastroenterology·L M HandyA Ferguson
Feb 1, 1997·Digestive Diseases and Sciences·J W ArndtE K Pauwels

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Citations

Apr 11, 2001·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·K K RichterM Hauer-Jensen
Dec 4, 2001·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·W Dörr, J H Hendry
Nov 10, 2000·International Journal of Radiation Oncology, Biology, Physics·N HovdenakM Hauer-Jensen
Dec 29, 1999·International Journal of Radiation Oncology, Biology, Physics·J WangM Hauer-Jensen
Jun 22, 2011·Cancer Treatment Reviews·Rachel J Gibson, Joanne M Bowen
Oct 1, 2010·The British Journal of Surgery·A MihaescuH Thorlacius
May 26, 2017·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·N S S KuikenWim J E Tissing

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