Association of high proliferation in adult T-cell leukemia cells with apoptosis, and expression of p53 protein in acute type ATL

Journal of Clinical and Experimental Hematopathology : JCEH
Jia WangFusayoshi Murata

Abstract

Proliferation, apoptosis and p53 protein expression in adult T-cell leukemia (ATL) cells were investigated. Twenty peripheral blood tissue specimens (PBTS) comprising 7 cases of acute type ATL, 7 cases of chronic type ATL and 6 other leukemias were examined by means of antigen retrieval and the polymer method employing anti-Ki67 antigen (MIB-1), anti-cleaved caspase-3, anti-single stranded DNA and three kinds of anti-p53 protein antibodies including DO7. Most acute and chronic cases of ATL included more than 10% MIB-1-positive proliferating leukemia cells and more than 1% cleaved caspase-3-positive apoptotic cells. Some cells which were positive for both MIB-1 and anti-cleaved caspase-3 antibody were observed in acute type ATL. Nuclear deposition of p53 protein labeled by DO7 was often found in acute type (p < 0.05). Within the medium-sized population of ATL cell nuclei, DO7-positive ATL cells had a smaller nuclear area factor (long axis x short axis) than DO7-negative ATL cells. A few proliferating ATL cells entered apoptosis, and the appearance of a subclone of ATL cells with nuclear deposition of p53 protein labeled by DO7 characterized acute type.

References

Sep 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K ChangM C Willingham
Jun 1, 1986·Journal of Clinical Pathology·K C GatterD Y Mason
Mar 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Allen M Gown, Mark C Willingham
Aug 19, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Kazuhisa HasuiFusayoshi Murata
Feb 10, 2004·The Journal of Biological Chemistry·James F Curtin, Thomas G Cotter

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