Abstract
Proliferation, apoptosis and p53 protein expression in adult T-cell leukemia (ATL) cells were investigated. Twenty peripheral blood tissue specimens (PBTS) comprising 7 cases of acute type ATL, 7 cases of chronic type ATL and 6 other leukemias were examined by means of antigen retrieval and the polymer method employing anti-Ki67 antigen (MIB-1), anti-cleaved caspase-3, anti-single stranded DNA and three kinds of anti-p53 protein antibodies including DO7. Most acute and chronic cases of ATL included more than 10% MIB-1-positive proliferating leukemia cells and more than 1% cleaved caspase-3-positive apoptotic cells. Some cells which were positive for both MIB-1 and anti-cleaved caspase-3 antibody were observed in acute type ATL. Nuclear deposition of p53 protein labeled by DO7 was often found in acute type (p < 0.05). Within the medium-sized population of ATL cell nuclei, DO7-positive ATL cells had a smaller nuclear area factor (long axis x short axis) than DO7-negative ATL cells. A few proliferating ATL cells entered apoptosis, and the appearance of a subclone of ATL cells with nuclear deposition of p53 protein labeled by DO7 characterized acute type.
References
Sep 1, 1992·AIDS Research and Human Retroviruses·H MasutaniY Yoshida
Jan 12, 1991·Lancet·T HattoriK Oizumi
Sep 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K ChangM C Willingham
Sep 1, 1987·Acta Pathologica Japonica·K HasuiM Tashiro
Jun 1, 1986·Journal of Clinical Pathology·K C GatterD Y Mason
Sep 1, 1987·Journal of Clinical Pathology·T SuchiA C Feller
Feb 1, 1986·Acta Pathologica Japonica·K Hasui, E Sato
Sep 1, 1994·Histopathology·S KitajimaT Arima
Apr 1, 1994·Leukemia & Lymphoma·H SuzushimaK Takatsuki
Mar 24, 1998·Japanese Journal of Cancer Research : Gann·M InoueM Yoshida
Apr 29, 1998·Journal of Virology·A TanimuraM Yoshida
Oct 18, 2000·Oncology Reports·K YoshiokaH Hibasami
Mar 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Allen M Gown, Mark C Willingham
Oct 24, 2002·Blood·Bertrand ArnulfOlivier Hermine
Aug 19, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Kazuhisa HasuiFusayoshi Murata
Feb 10, 2004·The Journal of Biological Chemistry·James F Curtin, Thomas G Cotter
May 25, 2004·Blood·Soo-Jin JeongCynthia A Pise-Masison
Citations
Aug 6, 2009·Apoptosis : an International Journal on Programmed Cell Death·Fang HeTong Wang
Jun 12, 2012·Acta Histochemica Et Cytochemica·Kazuhisa HasuiTakami Matsuyama
Sep 15, 2017·PLoS Pathogens·Leila SawadaMari Kannagi
Mar 27, 2009·Journal of Basic Microbiology·Rachna Nandwani, S S Dudeja
Jun 22, 2018·Oncology Letters·Zhangzhen ShiHongjun Li
Jun 20, 2014·Journal of Clinical and Experimental Hematopathology : JCEH·Yoichi SaitoMotohiro Takeya