Association of HMGB1, BRCA1 and P62 expression in ovarian cancer and chemotherapy sensitivity

Oncology Letters
Shouyong Li, Yanlei Wei

Abstract

The expression of high mobility group box 1 (HMGB1), breast cancer susceptibility gene 1 (BRCA1) and P62 in ovarian cancer was investigated to explore its association with chemotherapy sensitivity in ovarian cancer patients. Tumor tissues and para-carcinoma normal tissues of 60 ovarian cancer patients hospitalized in Department of Surgery in Dongying Hospital from June, 2012 to June, 2015 were collected. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect the mRNA expression levels of HMGB1, BRCA1 and P62 in tumor and para-carcinoma normal tissues. Moreover, immunohistochemistry was used to detect the protein expression of HMGB1, BRCA1 and P62 in tumor tissues and para-carcinoma normal tissues. The cancer tissue specimens were divided into the chemotherapy resistance group and sensitivity group through the in vitro resin droplet experiment to analyze the association of the expression of HMGB1, BRCA1 and P62 in epithelial ovarian cancer with chemotherapy resistance of patients. The RT-qPCR results showed that the expression of HMGB1, BRCA1 and P62 in ovarian cancer tissues at the mRNA level was significantly higher than that in para-carcinoma normal tissues. Immunohistochemical results showe...Continue Reading

Methods Mentioned

BETA
PCR

Software Mentioned

SPSS

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