Association of human CUL-1 and ubiquitin-conjugating enzyme CDC34 with the F-box protein p45(SKP2): evidence for evolutionary conservation in the subunit composition of the CDC34-SCF pathway

The EMBO Journal
J LisztwanW Krek

Abstract

In normal and transformed cells, the F-box protein p45(SKP2) is required for S phase and forms stable complexes with p19(SKP1) and cyclin A-cyclin-dependent kinase (CDK)2. Here we identify human CUL-1, a member of the cullin family, and the ubiquitin-conjugating enzyme CDC34 as additional partners of p45(SKP2) in vivo. CUL-1 also associates with cyclin A and p19(SKP1) in vivo and, with p45(SKP2), they assemble into a large multiprotein complex. In Saccharomyces cerevisiae, a complex of similar molecular composition (an F-box protein, a member of the cullin family and a homolog of p19(SKP1)) forms a functional E3 ubiquitin protein ligase complex, designated SCFCDC4, that facilitates ubiquitination of a CDK inhibitor by CDC34. The data presented here imply that the p45(SKP2)-CUL-1-p19(SKP1) complex may be a human representative of an SCF-type E3 ubiquitin protein ligase. We propose that all eukaryotic cells may use a common ubiquitin conjugation apparatus to promote S phase. Finally, we show that multiprotein complex formation involving p45(SKP2)-CUL-1 and p19(SKP1) is governed, in part, by periodic, S phase-specific accumulation of the p45(SKP2) subunit and by the p45(SKP2)-bound cyclin A-CDK2. The dependency of p45(SKP2)-p19(SK...Continue Reading

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Citations

Dec 18, 2004·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Sang Hyun Lee, Frank McCormick
Dec 3, 1999·Progress in Biophysics and Molecular Biology·K L Craig, M Tyers
Feb 19, 2002·International Journal of Radiation Oncology, Biology, Physics·Anu GuptaLing chun Chen
May 12, 2000·Gene·E T YehT Kamitani
Oct 18, 2002·Gene·Gennady P IlyinChristiane Guguen-Guillouzo
Nov 7, 2000·Current Opinion in Cell Biology·S V Ekholm, S I Reed
Jan 23, 1999·Current Opinion in Cell Biology·J M Peters
Apr 8, 1998·Current Opinion in Genetics & Development·W Krek
Jun 15, 1999·Molecular Medicine Today·M Ohh, W G Kaelin
Jan 3, 2003·Nature Cell Biology·Alexander HergovichWilhelm Krek
May 31, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ding WangChih-Pin Liu
Dec 21, 2010·Molecular & Cellular Proteomics : MCP·J Eugene LeeRaymond J Deshaies
Aug 3, 2001·The EMBO Journal·T KawakamiK Tanaka
Dec 3, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A R WillemsM Tyers
Dec 3, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·T TodaK Kominami
Sep 29, 1999·Genes & Development·J D SingerJ M Roberts
Mar 11, 2004·European Journal of Biochemistry·Tomoaki TanakaTetsu Kamitani
Mar 3, 2004·Journal of Virology·Syed Hamid AliJames A DeCaprio
Jul 19, 2006·Molecular and Cellular Biology·Alexander HergovichWilhelm Krek
Aug 2, 2006·Molecular and Cellular Biology·Abdelmajid SabileWilhelm Krek
Mar 10, 2001·Molecular and Cellular Biology·I LassotF Margottin-Goguet
Jun 22, 2002·Molecular and Cellular Biology·Binghui LiKristin T Chun

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