PMID: 8944713Nov 11, 1996Paper

Association of ICAM-1 with the cytoskeleton in rat alveolar epithelial cells in primary culture

The American Journal of Physiology
W W BartonR Paine

Abstract

Intercellular adhesion molecule-1 ICAM-1) is a transmembrane adhesion protein that is expressed constitutively on the apical surface of type I cells in vivo and on type II cells in vitro as they spread in culture, assuming type I cell-like characteristics. To investigate the possible interaction of ICAM-1 with the alveolar epithelial cell cytoskeleton, rat type II cells in primary culture were extracted with nonionic detergent, and residual ICAM-1 associated with the cytoskeletal remnants was determined using immunofluorescence microscopy, immunoprecipitation, and cell-based enzyme-linked immunosorbent assay. A large fraction of alveolar epithelial cell ICAM-1 remained associated with the cytoskeleton after detergent extraction, whereas two other transmembrane molecules, transferrin receptor and class II major histocompatibility complex, were completely removed. ICAM-1 was redistributed on the cell surface after the disruption of actin filaments with cytochalasin B, suggesting interaction with the actin cytoskeleton. In contrast, ICAM-1 was completely detergent soluble in rat pulmonary artery endothelial cells, human umbilical vein endothelial cells, and rat alveolar macrophages. The association of ICAM-1 with the alveolar epit...Continue Reading

Citations

Jun 16, 2001·Journal of Applied Physiology·J C BerriosR D Hubmayr
Jun 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Robert PaineSteven E Wilcoxen
Jun 28, 2011·American Journal of Respiratory Cell and Molecular Biology·Susan BuckleyDavid Warburton
Mar 14, 2001·American Journal of Physiology. Cell Physiology·H Lum, K A Roebuck
Apr 17, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Ehsan Y DavaniKeith R Walley

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