Association of macrophage and lymphocyte infiltration with outcome in canine osteosarcoma

Veterinary and Comparative Oncology
Sita S WithersR B Rebhun

Abstract

Immunotherapeutic strategies have shown promise for the treatment of canine osteosarcoma (cOSA). Very little is known about the immune microenvironment within cOSA, however, limiting our ability to identify potential immune targets and biomarkers of therapeutic response. We therefore prospectively assessed the disease-free interval (DFI) and overall survival time (ST) of 30 dogs with cOSA treated with amputation and six doses of adjuvant carboplatin. We then quantified lymphocytic (CD3+, FOXP3+) and macrophage (CD204+) infiltrates within the primary tumours of this cohort using immunohistochemistry, and evaluated their association with outcome. Overall, the median DFI and ST were 392 and 455 days, respectively. The median number of CD3+ and FOXP3+ infiltrates were 45.8 cells/mm2 (4.6-607.6 cells/mm2 ) and 8.5 mm2 (0-163.1 cells/mm2 ), respectively. The median area of CD204+ macrophages was 4.7% (1.3%-23.3%), and dogs with tumours containing greater than 4.7% CD204+ macrophages experienced a significantly longer DFI (P = 0.016). Interestingly, a significantly lower percentage of CD204+ macrophages was detected in cOSA arising from the proximal humerus compared to other appendicular bone locations (P = 0.016). Lymphocytic infiltr...Continue Reading

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Citations

Apr 5, 2020·Veterinary and Comparative Oncology·Matthew J AthertonNicola J Mason
Sep 16, 2019·BMC Cancer·Tanmay M ShekharChristine J Hawkins
Aug 14, 2020·Veterinary Pathology·Sara BellucoThierry Marchal
Jan 27, 2019·Veterinary and Comparative Oncology·Sita S WithersRobert B Rebhun
May 28, 2019·Veterinary Sciences·Kelly M MakielskiJaime F Modiano
Sep 17, 2020·Nature Reviews. Cancer·Amy K LeBlanc, Christina N Mazcko
Nov 10, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·Feng JiangJimei Wang

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