PMID: 3769924Oct 15, 1986Paper

Association of messenger RNA with the cytoskeletal framework in rat L6 myogenic cells

European Journal of Biochemistry
S K PramanikJ Bag

Abstract

The distribution of mRNA between the detergent-soluble and insoluble (cytoskeleton) fractions in rat L6 myoblast and myotube cells was examined. Approximately 85% of cytoplasmic mRNA in both myoblasts and myotubes was found associated with the cytoskeletal framework. The cytoskeleton-bound mRNA was present as polysomes. In contrast, the mRNA of the detergent-soluble fraction was not associated with ribosomes and was thus considered to be the repressed population. The association of mRNA with the cytoskeletal framework was not affected by treatments leading to dissociation of polysomes. Differential distribution of mRNA between the soluble and cytoskeleton-bound fractions was analyzed by in vitro translation. The mRNAs coding for polypeptides of molecular masses 40 kDa and 60 kDa were preferentially enriched in the soluble fraction. The nature of binding between mRNA and the cytoskeletal framework was examined following in vivo cross-linking of RNA and protein by irradiating muscle cells with ultraviolet light. It was observed that this treatment covalently linked RNA and the neighbouring protein moieties without any detectable damage to the cytoskeletal framework, as measured by the distribution of RNAs and proteins between the...Continue Reading

References

Feb 1, 1979·Cell·R Lenk, S Penman
Jan 1, 1978·Progress in Nucleic Acid Research and Molecular Biology·A A Preobrazhensky, A S Spirin
Aug 1, 1976·European Journal of Biochemistry·H R Pelham, R J Jackson
Oct 1, 1977·Cell·R C HardisonR Chalkley
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Nov 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S W Kwan, G Brawerman
Oct 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J C Mauck, H Green
Apr 1, 1983·Cell Biology International Reports·P NielsenH Trachsel
Jan 1, 1982·Cold Spring Harbor Symposia on Quantitative Biology
Jan 1, 1982·Cold Spring Harbor Symposia on Quantitative Biology·B R Brinkley
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R YenofskyG Brawerman
Jan 1, 1982·Annual Review of Biochemistry·U MaitraA Chaudhuri
Sep 1, 1981·Experimental Cell Research·W J van VenrooijR J Reinders
Nov 1, 1980·European Journal of Biochemistry·A J WagenmakersW J van Venrooij

❮ Previous
Next ❯

Citations

Jan 1, 1991·Cell and Tissue Research·D J Dix, B R Eisenberg
Jan 1, 1990·Molecular Biology Reports·C H de MoorH O Voorma
Jan 1, 1992·The American Journal of Physiology·B Russell, D J Dix
Apr 1, 1989·Experimental Cell Research·J H HeuijerjansW J Van Venrooij

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.