PMID: 19912782Feb 1, 1991Paper

Association of microtubule-associated protein (MAP1B) with growing axons in cultured hippocampal neurons

Molecular and Cellular Neurosciences
I Fischer, G Romano-Clarke

Abstract

Microtubule-associated protein 1B (MAP1B) is a major constituent of the neuronal cytoskeleton early in development. This protein is present in embryonic brain and is composed of two isoforms that are the result of differential phosphorylation. We examined the distribution of MAP1B during the differentiation of cultured hippocampal neurons and compared it to that of MAP2 and tubulin. We demonstrated by immunofluorescent doublestaining that MAP1B and MAP2 are colocalized in cell bodies and the minor processes of hippocampal neurons during the early stages of development, before the establishment of neuronal polarity. Later, when neurons acquire axonal and dendritic characteristics, MAP1B is sorted into growing axons, including the growth cone, whereas MAP2 is restricted to dendrites and cell bodies. Unlike tubulin, the localization of MAP1B in growing axons is not uniform. Rather, the protein is found concentrated in the distal portion. During later stages of development, the neurons extend a network of fasciculating axonal and dendritic neurites in which the segregation of MAP1B and MAP2 is maintained. However, the staining of MAP1B in mature neuronal cultures decreases in a pattern that resembles the decline of this protein dur...Continue Reading

References

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Citations

Feb 1, 1992·Developmental Biology·J A QianP Levitt
Oct 1, 1996·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·L J BoyneT B Shea
Mar 15, 2001·European Journal of Biochemistry·G Paglini, A Cáceres
Feb 1, 1996·The European Journal of Neuroscience·M S BushP R Gordon-Weeks
Apr 5, 2014·Developmental Neurobiology·David Villarroel-Campos, Christian Gonzalez-Billault
Jun 1, 2014·Experimental Eye Research·Bhavani S KowtharapuOliver Stachs
Aug 8, 2009·Molecular and Cellular Neurosciences·Fabienne E Poulain, André Sobel
Nov 7, 2003·Neurochemistry International·S SánchezF Wandosell
Jul 14, 2001·Molecular Biology of the Cell·C Gonzalez-BillaultA Cáceres
Jan 29, 2000·Microscopy Research and Technique·P R Gordon-Weeks, I Fischer
Dec 2, 2004·Cell Motility and the Cytoskeleton·Irina TintMark Black

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