Association of Mutations in the Basal Core Promoter and Pre-core Regions of the Hepatitis B Viral Genome and Longitudinal Changes in HBV Level in HBeAg Negative Individuals: Results From a Cohort Study in Northern Iran

Hepatitis Monthly
Sima BesharatReza Malekzadeh

Abstract

Although certain HBV mutations are known to affect the expression of Hepatitis e antigen, their association with HBV viral level or clinical outcomes is less clear. We evaluated associations between different mutations in the Basal Core promoter (BCP) and Pre-core (PC) regions of HBV genome and subsequent changes in HBV viral DNA level over seven years in a population of untreated HBeAg negative chronic hepatitis B (CHB) participants in Northeast of Iran. Participants in the current study were drawn from the Golestan Hepatitis B Cohort Study (GHBCS), a cohort of approximately 2590 HBsAg positive subjects (living in Gonbad city) embedded in the Golestan Cohort Study (GCS). At baseline, HBsAg was measured in all participants and revealed 2590 HBsAg positive cases. We randomly selected 304 participants who their blood sample were taken at both baseline and seven years later in follow-up and had not been treated for HBV during this time. HBV viral load were assessed at baseline and at year 7. The BCP and PC regions of the HBV DNA, at baseline, were amplified via hemi-nested PCR and sequenced by cycle sequencing. At year 7, liver stiffness was assessed by fibroscan; also, other parameters of liver disease were assessed following sta...Continue Reading

References

Sep 1, 1973·British Journal of Cancer·E MahboubiS Salmasizadeh
Mar 20, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·A M BozdayiO Uzunalimoğlu
Jul 26, 2006·BMC Gastroenterology·Zaigham AbbasSyed Adibul Hassan Rizvi
Mar 18, 2008·The Journal of General Virology·Saffie JammehPeter Karayiannis
Aug 13, 2008·Journal of the National Cancer Institute·Hwai-I YangUNKNOWN REVEAL-HBV Study Group
Oct 4, 2008·The New England Journal of Medicine·Jules L Dienstag
Feb 25, 2009·Journal of Medical Virology·Jong Won ChoiChae Yoon Chon
Apr 1, 2009·International Journal of Epidemiology·Akram PourshamsReza Malekzadeh
Nov 27, 2009·Virology Journal·Ashraf MohamadkhaniGhodratollah Montazeri
Jan 18, 2011·Journal of Hepatology·Stephanos J Hadziyannis
Sep 5, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Rania A TohmeBrian J McMahon
Nov 8, 2013·Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association·Amal AlghamdiAyman A Abdo

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Citations

Jun 3, 2016·Middle East Journal of Digestive Diseases·Shirin MoossaviAshraf Mohamadkhani
Jan 13, 2016·World Journal of Gastroenterology : WJG·Patrizia CaligiuriBianca Bruzzone
Mar 24, 2016·World Journal of Gastroenterology : WJG·Xiao-Ling WangYi Xiong

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Methods Mentioned

BETA
ELISA
PCR

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