Association of Mycobacterium Proteins with Lipid Droplets

Journal of Bacteriology
Richard M ArmstrongThomas C Zahrt

Abstract

Mycobacterium tuberculosis is a global pathogen of significant medical importance. A key aspect of its life cycle is the ability to enter into an altered physiological state of nonreplicating persistence during latency and resist elimination by the host immune system. One mechanism by which M. tuberculosis facilitates its survival during latency is by producing and metabolizing intracytoplasmic lipid droplets (LDs). LDs are quasi-organelles consisting of a neutral lipid core such as triacylglycerol surrounded by a phospholipid monolayer and proteins. We previously reported that PspA (phage shock protein A) associates with LDs produced in Mycobacterium In particular, the loss or overproduction of PspA alters LD homeostasis in Mycobacterium smegmatis and attenuates the survival of M. tuberculosis during nonreplicating persistence. Here, M. tuberculosis PspA (PspAMtb) and a ΔpspA M. smegmatis mutant were used as model systems to investigate the mechanism by which PspA associates with LDs and determine if other Mycobacterium proteins associate with LDs using a mechanism similar to that for PspA. Through this work, we established that the amphipathic helix present in the first α-helical domain (H1) of PspA is both necessary and suff...Continue Reading

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Citations

Jan 29, 2019·Frontiers in Cellular and Infection Microbiology·Rahul Kumar MauryaManju Y Krishnan
Feb 24, 2021·Archives of Microbiology·Mariana P LanfranconiHéctor M Alvarez
May 27, 2021·FEMS Microbiology Reviews·Ivy MallickStéphane Canaan
Jun 30, 2021·Journal of Medical Microbiology·Jacobus T R BrinkCarolina H Pohl
Sep 14, 2021·Journal of Bacteriology·Corelle A Z RokickiYasu S Morita

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