Abstract
Cdk5 exists in brain extracts in multiple forms, one of which is a macromolecular protein complex comprising Cdk5, neuron-specific Cdk5 activator p35nck5a and other protein components (Lee, K.-Y., Rosales, J. L., Tang, D., and Wang, J.H. (1996) J. Biol. Chem. 271, 1538-1543). The yeast two-hybrid system was employed to identify p35nck5a-interacting proteins from a human brain cDNA library. One of the isolated clones encodes a fragment of glial fibrillary acidic protein, which is a glial-specific protein. Sequence alignment revealed significant homology between the p35nck5a-binding fragment of glial fibrillary acidic protein and corresponding regions in neurofilaments. The association between p35nck5a and neurofilament medium molecular weight subunit (NF-M) was confirmed by both the yeast two-hybrid assay and direct binding of the bacteria-expressed proteins. The p35nck5a binding site on NF-M was mapped to a carboxyl-terminal region of the rod domain, in close proximity to the putative Cdk5 phosphorylation sites in NF-M. A region immediately amino-terminal to the kinase-activating domain in p35nck5a is required for its binding with NF-M. In in vitro binding assays, NF-M binds both monomeric p35nck5a and the Cdk5/p35nck5a complex...Continue Reading
References
Jan 1, 1991·International Review of Cytology·K H Fliegner, R K Liem
Jan 1, 1988·Annual Review of Biochemistry·P M Steinert, D R Roop
Aug 15, 1970·Nature·U K Laemmli
Nov 10, 1995·The Journal of Biological Chemistry·D TangJ H Wang
Apr 1, 1995·Molecular Biology of the Cell·J W HarperE Swindell
Jun 19, 1995·Neuroreport·M MatsushitaO Hatase
May 5, 1995·The Journal of Biological Chemistry·Z QiJ H Wang
May 15, 1995·Genes & Development·C J Sherr, J M Roberts
Mar 9, 1995·Nature·D O Morgan
Dec 1, 1994·Journal of Neurochemistry·E ElhananyH C Pant
Sep 29, 1994·Nature·L H TsaiE Harlow
Sep 29, 1994·Nature·J LewJ H Wang
Apr 4, 1994·FEBS Letters·K IshiguroT Uchida
Nov 19, 1993·Cell·J W HarperS J Elledge
Feb 1, 1993·Current Opinion in Cell Biology·R K Liem
Jan 1, 1995·Methods in Enzymology·P L Bartel, S Fields
Sep 8, 1995·Neuroscience Letters·B R SperberV M Lee
Aug 1, 1995·Molecular and Cellular Biochemistry·Z QiJ H Wang
Jan 19, 1996·The Journal of Biological Chemistry·K Y LeeJ H Wang
Dec 1, 1995·Current Opinion in Cell Biology·E Lees
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·M H LeeJ Massagué
Apr 1, 1996·Journal of Neurochemistry·S GuidatoC C Miller
Jun 14, 1996·The Journal of Biological Chemistry·D SunR K Liem
Sep 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·H C Pant, Veeranna
Apr 1, 1996·Genes & Development·M NikolicL H Tsai
Sep 1, 1996·Neuroscience·K TomizawaO Hatase
Dec 1, 1996·Journal of Neurochemistry·K HirookaO Hatase
Oct 25, 1996·Neuroscience Letters·J B LazaroN J Lamb
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·D TangJ H Wang
Jan 1, 1997·Neuron·T ChaeL H Tsai
Feb 28, 1997·The Journal of Biological Chemistry·R Y PoonT Hunter
May 9, 1997·The Journal of Biological Chemistry·D TangJ H Wang
May 16, 1997·The Journal of Biological Chemistry·J C BettsB H Anderton
Citations
Jul 9, 1999·Journal of Neurobiology·P GrantH C Pant
Oct 3, 2003·Biochemical and Biophysical Research Communications·Naoyuki HonmaShin-ichi Hisanaga
Jul 21, 1999·Neuroscience Research·K Y LeeR N Johnston
Mar 18, 2000·Gene·Y P ChingJ H Wang
Mar 23, 2000·European Journal of Biochemistry·T HiroseM Okada
Feb 13, 2001·The European Journal of Neuroscience·S KesavapanyC C Miller
Jun 20, 2001·Journal of Neurochemistry·M NeystatR E Burke
Jun 18, 2002·Journal of Neurochemistry·Wing-Yu FuNancy Y Ip
Sep 16, 2003·Molecular and Cellular Biology·Kamel KhaliliJennifer Gordon
Apr 12, 2011·Reviews in the Neurosciences·Alejandro López-TobónGloria Patricia Cardona-Gómez
Oct 27, 2010·Cellular and Molecular Life Sciences : CMLS·Lisheng HeRobert Z Qi
Aug 6, 2011·Progress in Neurobiology·Giulietta RiboldiStefania Corti
Jul 5, 2005·Journal of Neurochemistry·Ying-Shan ZhuShin-ichi Hisanaga
Feb 13, 2001·Neuron·S SasakiS Hirotsune
Feb 9, 2000·Chemistry & Biology·L MeijerG R Pettit
Apr 20, 2005·Advances in Protein Chemistry·Kathleen J GreenJonathan C R Jones
Nov 2, 2016·Molecular Biology of the Cell·Niranjana D AminHarish C Pant
Aug 28, 1999·Bioorganic & Medicinal Chemistry·M LegraverendV Favaudon
Mar 12, 2004·FEBS Letters·Robert Z QiJerry H Wang
Mar 15, 2003·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Marcel Ferrer-AlcónJesús A García-Sevilla
Mar 8, 2002·The Journal of Biological Chemistry·Yick-Pang ChingJerry H Wang
Feb 6, 1999·The Journal of Biological Chemistry·A I FletcherE L Stuenkel
Aug 1, 2000·The Journal of Biological Chemistry·X WangJ H Wang
Apr 5, 2000·The Journal of Biological Chemistry·G KusakawaS Hisanaga
Jun 27, 2002·The Journal of Biological Chemistry·Kai ChengNancy Y Ip
Dec 14, 2001·The Journal of Biological Chemistry·Dianbo QuRobert Z Qi
Feb 14, 2007·Glia·Yi HeYun Wang