PMID: 3745267Sep 1, 1986Paper

Association of nucleoplasmin with transcription products as revealed by immunolocalization in the amphibian oocyte

The Journal of Cell Biology
N A MoreauP Kubisz

Abstract

The oocyte nucleus of Pleurodeles waltlii contains a major 32,000-mol-wt acidic protein which is called nucleoplasmin. Rabbit antibodies were raised against total nuclear proteins from Pleurodeles oocytes. Affinity-purified antibodies directed against nucleoplasmin were prepared using antigens bound to nitrocellulose paper. The specificity of the antibody was controlled on two-dimensional electrophoretic gels of nuclear proteins. The intranuclear distribution of nucleoplasmin was analyzed by indirect immunofluorescence and the immunogold technique in light and electron microscopy. The antibody was tested on a spread of the nuclear content prepared in the presence of calcium, on the nuclear content spread in the presence of phalloidin so that an actin network appeared, and on a spread of nuclei from oocytes previously treated by actinomycin D. In all cases, nucleoplasmin appeared to be localized on the lampbrush loops, i.e., on the sites of transcription and, more specifically, on the ribonucleoprotein (RNP) particles; this protein was also associated with the RNP particles of the nuclear sap (free or inserted in the actin network). Nucleoplasmin was localized on large RNP particles that appeared when transcription was blocked. ...Continue Reading

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Citations

Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M B Roth, J G Gall
Mar 1, 1992·Molecular Biology of the Cell·A TsvetkovJ G Gall
Aug 1, 1989·Experimental Cell Research·N AngelierN F Lautredou
Jul 1, 1990·Experimental Cell Research·E N'Da, N Angelier
May 1, 1988·The Veterinary Clinics of North America. Small Animal Practice·D O'Brien
May 4, 1993·Biochemistry·A R SburlatiS L Berger
Nov 1, 1990·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Nicole MoreauNicole Lautredou

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