Association of occlusal interference-induced masseter muscle hyperalgesia and P2X3 receptors in the trigeminal subnucleus caudalis and midbrain periaqueductal gray

Neuroreport
Shuzhen SunQingting Wu

Abstract

P2X3 receptor plays a role in nociception transmission of orofacial pain in temporomandibular disorder patients. A previous study found that P2X3 receptors in masseter muscle afferent neurons and the trigeminal ganglia were involved in masseter muscle pain induced by inflammation caused by chemical agents or eccentric muscle contraction. In this study, we attempted to investigate changes in P2X3 receptors in the trigeminal subnucleus caudalis (Vc) and midbrain periaqueductal gray (PAG) in relation to the hyperalgesia of masseter muscles induced by occlusal interference. Experimental occlusal interference by crown application was established in 30 rats and another 30 rats were treated as sham controls. On days 1, 3, 7, 14, and 28 after crown application, the mechanical pain threshold was examined by von-Frey filaments. The expression of the P2X3 receptor in Vc and PAG was investigated by immunohistochemistry and quantitative PCR. We found that mechanical pain threshold of bilateral masseter muscles decreased significantly after occlusal interference, which remained for the entire experimental period. The mRNA expression of the P2X3 receptor increased significantly and the number of P2X3R-positive neurons increased markedly in Vc...Continue Reading

References

Jul 1, 1989·The Journal of Prosthetic Dentistry·P KirveskariT Jämsä
Aug 18, 1999·Electrophoresis·R A WorthingtonJ A Barden
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May 7, 2013·The Journal of Pain : Official Journal of the American Pain Society·Ye CaoBarry J Sessle
Jul 10, 2014·Current Medicinal Chemistry·G Burnstock

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Citations

May 23, 2019·Purinergic Signalling·Bruna de Melo AquinoMaria Claudia Goncalves Oliveira-Fusaro
Sep 22, 2017·Pain Research & Management : the Journal of the Canadian Pain Society = Journal De La Société Canadienne Pour Le Traitement De La Douleur·Annalisa MonacoEleonora Ortu
Nov 4, 2017·Cranio : the Journal of Craniomandibular Practice

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