Association of PAI-1 4G/5G and -844G/A gene polymorphisms and changes in PAI-1/tissue plasminogen activator levels in myocardial infarction: a case-control study

Genetic Testing and Molecular Biomarkers
Nesrine AbboudTouhami Mahjoub

Abstract

Myocardial infarction (MI) is induced by acquired and inherited risk factors, including the plasminogen activator inhibitor-1 (PAI-1) -844G/A and -675G/A (4G/5G) gene variants. The aim of this study was to investigate the association between PAI-1-844G/A and 4G/5G polymorphisms and changes in PAI-1 and tissue plasminogen activator (tPA) levels in MI in a Tunisian population. This was a case-control study involving 305 patients with MI and 328 unrelated healthy controls. PAI-1 genotyping was done by polymerase chain reaction-restriction fragment length polymorphism (RFLP) (-844G/A) or by polymerase chain reaction-allele specific amplification. PAI-1 and tPA levels were assayed by serological assays. In contrast to tPA levels, mean plasma PAI-1 antigen levels were higher in cases than in control subjects. The elevation in PAI-1 levels was more pronounced in -844A and 4G allele carriers. Significantly higher frequencies of (mutant) 4G and -844A alleles and 4G/4G and -844A/-844A genotypes, and corresponding lower frequencies of (wild-type) 5G and -844G alleles and 5G/5G and -844G/-844G genotypes were seen in patients than in controls. Increased prevalence of 4G/-844A and decreased prevalence of 5G/-844G haplotypes were seen in pati...Continue Reading

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Citations

Mar 20, 2015·Disease Markers·Ilian Janet García-GonzálezJorge Ramón Padilla-Gutiérrez
Sep 1, 2015·PloS One·Marquitta J WhiteScott M Williams
Nov 20, 2016·The Journal of International Medical Research·Xia XuHongyu Zhang
Mar 26, 2013·American Journal of Reproductive Immunology : AJRI·Kalthoum MagdoudTouhami Mahjoub
Dec 17, 2014·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Maryam SakhtehMohammad Faranoush
Jan 9, 2021·Frontiers in Cardiovascular Medicine·Machteld Sillen, Paul J Declerck

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
electrophoretic mobility shift

Software Mentioned

Plus Haplotype
SPSS
HLA Stat2000

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