PMID: 8593280Jan 31, 1996Paper

Association of polyene antibiotics with sterol-free lipid membranes: I. Hydrophobic binding of filipin to dimyristoylphosphatidylcholine bilayers

Biochimica Et Biophysica Acta
J MilhaudA Blume

Abstract

The interaction of filipin III with multilamellar vesicles (MLV) of dimyristoylphosphatidylcholine (DMPC ) was studied by four complementary methods leading to the following results: (1) The modifications of the filipin dichroic spectrum, by adding preformed fluid DMPC MLV, provide evidence of a saturable association with the stoichiometry DMPC/filipin = 4.2 +/- 0.5, constant between 24 and 35 degrees Celsius. (2) Thermograms obtained by differential scanning calorimetry (DSC) on mixtures where filipin is incorporated during the formation of MLV exhibit a high-temperature tail the more marked the higher the filipin content and some structures at temperatures which depend on this content. The corresponding evolution with the temperature of the CD spectra reveals that the characteristic bound filipin spectrum appears at the temperature at which a structure emerges. (3) Titration calorimetry measurements reveal that the association process is exothermic in the temperature range of the DSC endotherms in agreement with the filipin-induced ordering of the lipid chains, previously established by 2H-NMR in the same temperature range (Milhaud et al.(1989) Eur. Biophys. J. 17, 151-158). A discussion of the relevancy of this exothermicity...Continue Reading

References

Sep 24, 1991·Biochemistry·J Seelig, P Ganz
Mar 22, 1988·Biochimica Et Biophysica Acta·L MaurinA Bienvenüe
Sep 27, 1973·Biochimica Et Biophysica Acta·T W Tillack, S C Kinsky
Oct 1, 1993·Journal of Biomolecular Structure & Dynamics·A R Balakrishnan, K R Easwaran

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Citations

Jul 24, 2007·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Hélder A SantosKyösti Kontturi
Jul 30, 2014·Chemistry and Physics of Lipids·Evgeny G ChulkovOlga S Ostroumova
Aug 26, 2004·Biochimica Et Biophysica Acta·Malika LarabiMonique Cheron
Jun 2, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Xueting JinHoward S Kruth
Aug 7, 2001·Journal of Neuropathology and Experimental Neurology·T MoriM J Mullan

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