PMID: 9188800May 22, 1997Paper

Association of polyene antibiotics with sterol-free lipid membranes. II. Hydrophobic binding of nystatin to dilauroylphosphatidylcholine bilayers

Biochimica Et Biophysica Acta
J MilhaudE J Dufourc

Abstract

Interaction of nystatin A1 with multilamellar vesicles (MLV) of dilauroylphosphatidylcholine (DLPC), observed either by adding nystatin to preformed MLV (mixtures I) or by incorporating it during the formation of vesicles (mixtures II, inner lamellas of MLV in contact with nystatin) was investigated for 0.002 < or = nystatin/DLPC = R(A) < or = 0.20, by four complementary methods. The main results were: (i) Ultraviolet absorption and circular dichroism (CD) spectra of mixtures I revealed the occurrence of a saturable association with a stoichiometry (R(A) = 0.007 +/- 0.002) constant between 3 and 33 degrees C. (ii) By differential scanning calorimetry, thermograms of the two types of mixtures were similar only when water was in great excess. In the opposite (e.g., (H2O)/(DLPC) = R(W) < or = 300), mixture II thermograms displayed two features, upshifted by about 6.5 degrees C with respect to the sharp peak observed with mixture I, resembling those obtained for pure DLPC when the low-temperature phase was the subgel phase. For this R(W), the nystatin absolute concentrations were those for which nystatin form superaggregates as revealed by the nystatin CD spectra. It is proposed that these superaggregates are excluded from the inte...Continue Reading

References

Apr 26, 1991·Biochimica Et Biophysica Acta·R P PetersonS C Hartsel
Oct 31, 1983·Biochemical and Biophysical Research Communications·J MazerskiE Borowski

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