Association of protein kinase A type I with detergent-resistant structures of mammalian sperm cells

Molecular Reproduction and Development
J MoosP Hozák

Abstract

The finding that flagellar movement in detergent-permeabilized sperm cells is restored when Mg ATP and cAMP are added implicated detergent-resistant protein kinase A (PKA) in the regulation of sperm motility. It is widely believed that only the PKA regulatory subunit RII can associate with the cytoskeleton and/or organelles. In this paper we used monoclonal antibodies against the PKA catalytic subunit and RI subunit and demonstrated that PKA type I is also associated with the sperm cytoskeleton. To our knowledge, this is the first report showing anchored PKA type I. This association was found in sperm of nonrodent mammalian species and, to a lesser extent, also in mouse sperm. The PKA catalytic subunit is bound to the cytoskeleton secondarily via its complex with the regulatory subunit. The detergent-resistant complexes of RI and catalytic subunits localize predominantly to the flagellum. Ultrastructural immunogold labeling revealed the association of detergent-resistant PKA type I with outer dense fibers (ODF) and the fibrous sheath (FS) but not with microtubules. This location is consistent with a proposed role of PKA in regulation of FS sliding on underlying ODF.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Oct 1, 1992·Molecular Reproduction and Development·J MoosJ Drahorád
Jan 1, 1989·Archives of Andrology·C B Lindemann, K S Kanous
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Jan 1, 1994·Cell Motility and the Cytoskeleton·J T San Agustin, G B Witman
Nov 1, 1995·Trends in Biochemical Sciences·Z Songyang, L C Cantley

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Citations

Sep 18, 2009·Human Reproduction Update·Claire L BorgMoira K O'Bryan
May 25, 2012·Biology of Reproduction·Valeria S Mouguelar, Gabriela Coux
Dec 22, 1999·The American Journal of Physiology·A G PratH F Cantiello
Oct 8, 2020·International Journal of Molecular Sciences·Nailis SyifaTzu-Hua Wu
Aug 9, 2002·Developmental Biology·Kiyoshi MikiEdward M Eddy

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