PMID: 8609374Feb 1, 1996Paper

Association of protein kinase-C-alpha with cytoplasmic vesicles in melanoma cells

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
J TimarK V Honn

Abstract

In B16a melanoma cells, protein kinase-C-alpha (PKC alpha) is immunomorphologically associated with cytoplasmic vesicles in addition to the previously observed locations (plasma membrane, cytoskeleton, nucleus), as detected with monoclonal antibody (MAb) MC3a. Subcellular fractionation indicated that the authentic 80-KD protein as well as PKC activity can be detected in several particulate fractions except for L2, which contains dense lysosomes. The highest PKC activity is associated with the cytosol-ultralight vesicles and the L1 fraction (containing plasma membrane, endosomes, and the Golgi apparatus). Both of these fractions contained the fluid-phase endocytosis marker peroxidase, indicating that PKC alpha, in addition to other subcellular structures, is most probably associated with endosomal membranes in B16a melanoma cells.

Citations

Oct 30, 1998·Cellular Signalling·W S Liu, C A Heckman
Apr 2, 2004·Melanoma Research·Michael M Lahn, Karen L Sundell

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