Association of rat C-reactive protein and other pentraxins with rat lipoproteins containing apolipoproteins E and A1

Biochemistry
R A SchwalbeG L Nelsestuen

Abstract

C-Reactive protein (CRP) is a member of the pentraxin family of proteins, ubiquitous components of animal serum. This study suggests that, in serum, rat CRP is complexed with lipoprotein and may interact directly with apolipoprotein E. When mixed with diluted rat serum, radiolabeled rat CRP showed a slightly higher sedimentation coefficient (about 15%) than that of the free protein. Elimination of calcium or addition of O-phosphorylethanolamine (O-PE), a low molecular weight compound that binds tightly to rat CRP in a calcium-dependent manner, abolished this difference. Adsorption of rat serum on a rat CRP affinity gel and elution with PE resulted in the isolation of material containing high levels of apolipoproteins E and A1. The affinity-purified preparation interacted with rat CRP and altered the sedimentation coefficient of the latter to the value observed in whole serum. Conversely, rat CRP increased the sedimentation coefficient of the major component of the affinity-purified material or to diluted rat serum, human serum amyloid P (SAP) and hamster female protein (FP), two other members of the pentraxin protein family, also had slightly higher sedimentation coefficients. In contrast, human CRP showed no evidence of an int...Continue Reading

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J E Coe
Apr 1, 1990·The Journal of Experimental Medicine·J E Coe, M J Ross
Jan 1, 1991·Immunologic Research·J M Kilpatrick, J E Volanakis
Sep 1, 1991·Immunobiology·V Kolb-Bachofen
Jan 1, 1988·Methods in Enzymology·M Skinner, A S Cohen
May 1, 1987·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·U SaxenaS Mookerjea
Jan 1, 1986·Methods in Enzymology·S C RallR W Mahley
Sep 1, 1985·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·U SaxenaS Mookerjea
Jul 1, 1985·The Journal of Clinical Investigation·J E Coe, M J Ross
Feb 1, 1971·Proceedings of the Society for Experimental Biology and Medicine·J E Volanakis, M H Kaplan
Apr 28, 1970·Biochimica Et Biophysica Acta·I Kushner, J A Somerville
Aug 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·E C Gotschlich, G M Edelman
Feb 1, 1984·The Journal of Experimental Medicine·I F RoweM B Pepys
Jan 1, 1983·Methods in Enzymology·N Jentoft, D G Dearborn
Jan 1, 1982·Annals of the New York Academy of Sciences·J J Morley, I Kushner
Jan 1, 1982·Annals of the New York Academy of Sciences·E C GotschlichE Oliveira
Jan 1, 1982·Annals of the New York Academy of Sciences·M L BaltzM B Pepys
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W J StrittmatterA D Roses
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W J StrittmatterA D Roses

❮ Previous
Next ❯

Citations

Oct 19, 2006·Annals of Human Genetics·Leslie A LangeDonald W Bowden
Dec 13, 2005·Proteomics·Leonardo RossiMonica C Panelli
Nov 6, 2008·Journal of Thrombosis and Haemostasis : JTH·S I van LeuvenE S Stroes
Apr 18, 2000·Electrophoresis·S CelliR H Kelly
Dec 13, 2006·The American Journal of Clinical Nutrition·Giovanni RavagliaChristopher Patterson
May 23, 2019·Frontiers in Immunology·Asmita Pathak, Alok Agrawal
Jul 12, 2021·Biological Psychiatry·Joris R DelangheMarc L De Buyzere

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE Phenotypes

Apolipoprotein E (APOE) is a protein involved in fat metabolism and associated with the pathogenesis of Alzheimer's disease and cardiovascular disease. Here is the latest research on APOE phenotypes.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.