Association of serum levels of antibodies against MMP1, CBX1, and CBX5 with transient ischemic attack and cerebral infarction

Oncotarget
Hao WangTakaki Hiwasa

Abstract

Transient ischemic attack (TIA) is a predictor for cerebral infarction (CI), and early diagnosis of TIA is extremely important for the prevention of CI. We set out to identify novel antibody biomarkers for TIA and CI, and detected matrix metalloproteinase 1 (MMP1), chromobox homolog 1 (CBX1), and chromobox homolog 5 (CBX5) as candidate antigens using serological identification of antigens by recombinant cDNA expression cloning (SEREX) and Western blotting to confirm the presence of serum antibodies against the antigens. Amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) revealed that serum antibody levels were significantly higher in patients with TIA or acute-phase CI (aCI) compared with healthy donors (P< 0.01). Spearman's correlation analysis and multivariate logistic regression analysis demonstrated that levels of anti-MMP1, anti-CBX1, and anti-CBX5 antibodies were associated with age, cigarette-smoking habits, and blood pressure. Thus, serum levels of antibodies against MMP1, CBX1, and CBX5 could potentially serve as useful tools for diagnosing TIA and predicting the onset of aCI.

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Citations

Jun 1, 2018·Neurologia Medico-chirurgica·Yoichi YoshidaYasuo Iwadate
Oct 26, 2018·Bulletin of Experimental Biology and Medicine·N V NizyaevaA I Shchegolev
Dec 12, 2020·Cancer Science·Sohei KobayashiKazuyuki Matsushita
May 29, 2021·Frontiers in Oncology·Jian ZhouTang Liu
Nov 7, 2021·Scientific Reports·Masaaki KubotaYasuo Iwadate

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Methods Mentioned

BETA
electrophoresis
assay-linked immunosorbent assay
AlphaLISA

Software Mentioned

Basic Local Alignment Search Tool
SEREX
AlphaLISA
GraphPad Prism

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