Abstract
Three genetic variants in the promoter of SPP1 (secreted phosphoprotein 1) gene have been reported to affect transcriptional activity of SPP1, thus conferring an increased risk for some diseases. To testify if these variants are associated with risk of hip osteoarthritis (OA) as well, we performed a case-control study including 389 hip OA patients and 315 healthy controls. Genotypes of SPP1 were determined by DNA sequencing, and differential expressions of SPP1 in relation with genotypes were evaluated by RT-PCR and ELISA. The results showed that rs17524488 (delG>insG) increased the risk of hip OA, with the adjusted OR 1.48 (95% CI 1.18-1.85, P<0.01) for risk allele insG, 1.90 (95% CI 1.35-2.66, P<0.01) for delG/insG and 2.04 (95% CI 1.20-3.49, P<0.01) for insG/insG respectively. However, as for rs11730582 (T>C), the adjusted ORs were 1.18 (95% CI 0.94-1.49, P=0.148) for allele C, 1.26 (95% CI 0.90-1.75, P=0.158) for TC, and 1.31 (95% CI 0.77-2.24, P=0.293) for CC, indicating no association of rs11730582 with hip OA risk. The variant rs28357094 was not observed in the tested subjects. Furthermore, the delG/insG and insG/insG genotypes of rs17524488 both correlated with higher levels of SPP1 expression in articular cartilage (P<...Continue Reading
References
Dec 1, 1992·The Anatomical Record·M D McKeeA Nanci
Aug 15, 1998·Arthritis and Rheumatism·D T Felson, Y Zhang
Aug 26, 1998·Acta histochemica·T NakaseT Ochi
Dec 31, 1998·Arthritis and Rheumatism·C MelchiorriA Facchini
Aug 11, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·O PulligB Swoboda
Mar 10, 2001·Osteoarthritis and Cartilage·I MartinM Heberer
Mar 27, 2001·Arthritis and Rheumatism·M G AtturA R Amin
Apr 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kenji YumotoMasaki Noda
Jan 17, 2003·Molecular Biology of the Cell·M A ChellaiahK A Hruska
Jan 7, 2004·Biochemical and Biophysical Research Communications·Satoshi MochidaKenji Fujiwara
Oct 14, 2004·Physiological Genomics·Francesca GiacopelliRoberto Ravazzolo
Feb 5, 2005·Arthritis and Rheumatism·S D'AlfonsoP Momigliano-Richiardi
Mar 12, 2005·The Journal of Clinical Investigation·Guangwu XuJingwu Z Zhang
Mar 16, 2005·Lancet·Paul A Dieppe, L Stefan Lohmander
Jun 7, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Rieko YagiWalter E Horton
Jul 13, 2005·Molecular Immunology·Tina HummelshojArne Svejgaard
Nov 1, 2006·Arthritis and Rheumatism·Thomas AignerEckart Bartnik
Mar 31, 2007·Bone·Gerald J AtkinsHoward A Morris
May 7, 2008·Molecular Carcinogenesis·Julia SchultzManfred Kunz
Oct 28, 2008·Cytokine & Growth Factor Reviews·Kathryn X Wang, David T Denhardt
Feb 17, 2009·Clinical Biochemistry·Sittisak HonsawekPongsak Yuktanandana
Sep 15, 2009·Osteoarthritis and Cartilage·S G GaoG H Lei
Jan 12, 2010·Osteoarthritis and Cartilage·C KarlssonJ Ringe
Jan 16, 2010·Journal of Human Genetics·Jin Dai, Shiro Ikegawa
Feb 11, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Chia-Chu LiuMing-Tsang Wu
Mar 12, 2010·Oral Oncology·Yu-Wei ChiuShou-Yen Kao
Nov 17, 2010·Nature Reviews. Rheumatology·Ana M Valdes, Tim D Spector
Apr 20, 2011·Pathology International·Toshimitsu Uede
Jul 19, 2011·Human Immunology·Nadia BarizzoneSandra D'Alfonso
Dec 14, 2011·The Journal of Biological Chemistry·Miguel OteroMary B Goldring
Dec 20, 2011·Bone·Kay Chapman, Ana M Valdes
Oct 18, 2012·BMC Cancer·Fujun ZhaoGuoxin Zhang
Jan 8, 2013·Arthritis Research & Therapy·Yongqing JiangChangwei Zhou
Feb 19, 2013·Expert Reviews in Molecular Medicine·Louise N Reynard, John Loughlin