Association of T cell dysfunction with the presence of IgG autoantibodies on CD4+ lymphocytes in haemophilia patients; results of a 10-year study

Clinical and Experimental Immunology
V DanielG Opelz

Abstract

HIV induces progressive dysfunction followed by numerical depletion of CD4+ lymphocytes. IgG autoantibodies and gp 120-containing immune complexes have been implicated in the pathogenesis of AIDS. We carried out a longitudinal study in 19 HIV- and 72 HIV+ haemophilia patients over a 10-year period in order to investigate a possible relationship between the occurrence of autoantibodies and CD4+ lymphocyte changes. IgM, IgG, C3d and gp120 on the surface of CD4+ lymphocytes were determined in heparinized whole blood with flow cytometry and double-fluorescence. The in vitro response of autoantibody-coated cells was tested in cell cultures with concanavalin A (Con A), phytohaemagglutinin (PHA), pokeweed mitogen (PWM) anti-CD3 MoAb or pooled allogeneic stimulator cells (MLC). After a 10-year follow up, 12 of 71 HIV+ and 16 of 19 HIV- haemophilia patients showed no evidence of immunoglobulins on circulating CD4+ lymphocytes. HIV- haemophilia patients without autoantibodies had CD4+ and CD8+ cell counts in the normal range (957+/-642/microliters and 636+/-405/microliters) and normal T cell responses in vitro (mean relative response (RR) > or = 0.7). In contrast, HIV+ haemophilia patients showed immunological abnormalities which were as...Continue Reading

Citations

Jan 1, 2014·BioResearch Open Access·Volker DanielGerhard Opelz
Feb 23, 2002·Molecular Immunology·Cornelia SpethPhilippe Gasque
Dec 17, 2010·Immunologic Research·Haixiang JiangMichael M Frank
Apr 9, 2013·The Journal of Allergy and Clinical Immunology·Johann GreilAndreas E Kulozik
Mar 9, 2005·Immunology Letters·Zoltán BánkiManfred P Dierich
Dec 5, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kathrin ZimmermannAnnette Oxenius

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