Association of telomerase activity with radio- and chemosensitivity of neuroblastomas.

Radiation Oncology
Simone WesbuerBurkhard Greve

Abstract

Telomerase activity compensates shortening of telomeres during cell division and enables cancer cells to escape senescent processes. It is also supposed, that telomerase is associated with radio- and chemoresistance. In the here described study we systematically investigated the influence of telomerase activity (TA) and telomere length on the outcome of radio- and chemotherapy in neuroblastoma. We studied the effects on dominant negative (DN) mutant, wild type (WT) of the telomerase catalytic unit (hTERT) using neuroblastoma cell lines. The cells were irradiated with 60Co and treated with doxorubicin, etoposide, cisplatin and ifosfamide, respectively. Viability was determined by MTS/MTT-test and the GI50 was calculated. Telomere length was measured by southernblot analysis and TA by Trap-Assay. Compared to the hTERT expressing cells the dominant negative cells showed increased radiosensitivity with decreased telomere length. Independent of telomere length, telomerase negative cells are significantly more sensitive to irradiation. The effect of TA knock-down or overexpression on chemosensitivity were dependent on TA, the anticancer drug, and the chemosensitivity of the maternal cell line. Our results supported the concept of tel...Continue Reading

References

Apr 1, 1997·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J W Shay, S Bacchetti
Aug 12, 1999·Annals of Oncology : Official Journal of the European Society for Medical Oncology·C PorembaB Dockhorn-Dworniczak
Sep 30, 1999·Nature Medicine·W C HahnR A Weinberg
Jul 13, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·C PorembaB Dockhorn-Dworniczak
Jul 13, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M Meyerson
Aug 30, 2000·Virchows Archiv : an International Journal of Pathology·K DhaeneR Parwaresch
Dec 6, 2000·The Journal of Experimental Medicine·F A GoytisoloM A Blasco
Feb 22, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·H M KatzensteinS L Cohn
Dec 20, 2002·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·Andreas SchuckNormann Willich
May 22, 2003·Cancer Letters·Gabriele Saretzki
Jan 6, 2004·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·Dirk NeuhofKlaus-Josef Weber
Jun 1, 2005·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·Hans Theodor EichBarbara Hero
Jul 11, 2006·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·Thorsten SimonFrank Berthold
Jul 28, 2006·Cold Spring Harbor Symposia on Quantitative Biology·M Armanios, C W Greider
Aug 19, 2006·Anti-cancer Drugs·Claudia Lanvers-KaminskyJoachim Boos
Nov 23, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Anna GenescàLaura Tusell
Jul 3, 2007·British Journal of Pharmacology·P Phatak, A M Burger
Jul 3, 2007·Toxicology Letters·M CastellaJ Surralles
Jan 31, 2008·British Journal of Cancer·J W Shay, W N Keith
Feb 8, 2008·Nature Reviews. Cancer·Calvin B Harley
Mar 20, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Barbara HeroFrank Berthold
May 3, 2008·Cancer Research·Manjula AgarwalTej K Pandita
May 31, 2008·Nature Reviews. Cancer·Michael BaumannRichard Hill
Oct 10, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Christopher JansonNina S Kadan-Lottick
Jan 6, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Calin O MarianRobert M Bachoo
Apr 8, 2010·International Journal of Oncology·Marina ArifinKeiko Hiyama

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Citations

Oct 16, 2012·Molecular Neurobiology·Sylvain FerrandonDelphine Poncet
Aug 14, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sharada D VangipuramWilliam D Lyman
Oct 22, 2013·Radiation and Environmental Biophysics·Michael OrthClaus Belka
Jan 5, 2013·Mutation Research·Evelyn LamyVolker Mersch-Sundermann
May 15, 2015·Journal of the National Cancer Institute·Kishore B ChallagundlaMuller Fabbri
Feb 4, 2014·Mutation Research. Reviews in Mutation Research·Grace ShimLaure Sabatier
Jul 31, 2014·Journal of Cancer Research and Clinical Oncology·B H LasterJ Kost
May 31, 2016·Genes·Yucheng Xu, Amir Goldkorn
Sep 23, 2016·European Journal of Medicinal Chemistry·Philip A WaghornKatherine A Vallis
Feb 2, 2011·Molecular BioSystems·Jana MajerskáJiří Fajkus
Feb 13, 2019·International Journal of Radiation Biology·Kumari AnujaBirendranath Banerjee
Jun 14, 2019·Frontiers in Molecular Neuroscience·Hisham F BahmadWassim Abou-Kheir
Nov 15, 2020·Journal of Experimental & Clinical Cancer Research : CR·Zongjuan LiLijuan Zou
Jan 28, 2014·Cancer Cell International·Zheng LiYunfeng Zhou

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Methods Mentioned

BETA
X-ray
Assay
PCR
electrophoresis
transfection
antisense oligonucleotides

Software Mentioned

Sigma Plot

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