Association of the components of the binary toxin from Bacillus sphaericus in solution and with model lipid bilayers

Biochemical and Biophysical Research Communications
Panadda BoonsermJaume Torres

Abstract

We show herein that interaction in aqueous solution of the two components of binary toxin from Bacillus sphaericus, BinA and BinB, leads to a dramatic conformational change, from beta turns or random coil, to beta structure. Also, either BinA or BinB separately or their equimolar mixture, interact with lipid bilayers resulting in further conformational changes. Upon membrane association, the change in conformation observed for BinA or BinB separately is different from that observed when the proteins are combined, indicating that proper folding depends on the presence of the complementary subunit. We also show, in contrast to previous reports, that BinB, but not BinA, is able to insert in model neutral lipid monolayers.

References

Dec 1, 1990·Canadian Journal of Microbiology·E W DavidsonC Berry
Jun 1, 1987·Applied and Environmental Microbiology·A H Broadwell, P Baumann
Nov 1, 1987·Canadian Journal of Microbiology·E W DavidsonA L Bieber
Jul 20, 1993·Journal of Molecular Biology·B Rost, C Sander
May 1, 1997·Journal of Invertebrate Pathology·C CokmusK Cooper
Jun 23, 1998·Quarterly Reviews of Biophysics·L K Tamm, S A Tatulian
Mar 14, 2003·Trends in Biochemical Sciences·Jaume TorresMontserrat Samsó
Jan 1, 1996·Annual Review of Entomology·J F CharlesA Delécluse
Sep 4, 2004·Biophysical Journal·Canay Ege, Ka Yee C Lee
Feb 15, 2005·Journal of Invertebrate Pathology·A W SmithJ P Allen

❮ Previous
Next ❯

Citations

Aug 15, 2009·Current Microbiology·Suweeraya LimpanawatPanadda Boonserm
Feb 7, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Kanokporn SrisucharitpanitPanadda Boonserm
Oct 23, 2010·Journal of Invertebrate Pathology·Chontida TangsongcharoenBoonhiang Promdonkoy
Mar 19, 2008·Micron : the International Research and Review Journal for Microscopy·Janaina Viana de MeloMaria Helena Neves Lobo Silva-Filha
Sep 27, 2015·Journal of Invertebrate Pathology·Hataikarn LekakarnPanadda Boonserm
Mar 29, 2008·Protein Science : a Publication of the Protein Society·Siok Wan GanJaume Torres
Aug 27, 2013·Insect Biochemistry and Molecular Biology·Avinash KaleVinay Kumar
May 11, 2015·Journal of Invertebrate Pathology·Chontida TangsongcharoenPanadda Boonserm
Dec 6, 2011·Journal of Invertebrate Pathology·Colin Berry
Dec 17, 2009·FEMS Microbiology Letters·Kamonnut SingkhamananPanadda Boonserm
Feb 2, 2020·Applied and Environmental Microbiology·Nathaly Alexandre NascimentoMaria Helena Neves Lobo Silva-Filha
Aug 27, 2021·Toxins·Maria Helena Neves Lobo Silva-FilhaAlejandra Bravo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.