PMID: 9548719May 23, 1998Paper

Association of the myosin-binding subunit of myosin phosphatase and moesin: dual regulation of moesin phosphorylation by Rho-associated kinase and myosin phosphatase

The Journal of Cell Biology
Y FukataK Kaibuchi

Abstract

The small GTPase Rho is believed to regulate the actin cytoskeleton and cell adhesion through its specific targets. We previously identified the Rho targets: protein kinase N, Rho-associated kinase (Rho-kinase), and the myosin-binding subunit (MBS) of myosin phosphatase. We found that in MDCK epithelial cells, MBS accumulated at the tetradecanoylphorbol-13-acetate (TPA)-induced membrane ruffling area, where moesin, a member of the ERM (ezrin, radixin, and moesin) family, was localized. Neither membrane ruffling nor an accumulation of moesin and MBS at the free-end plasma membrane was induced when MDCK cells were stimulated with TPA after the microinjection of C3, which ADP-ribosylates and inactivates Rho. MBS was colocalized with moesin at the cell-cell contact sites in MDCK cells. We also found that moesin was coimmunoprecipitated with MBS from MDCK cells. Recombinant MBS interacted with the amino-terminal domains of moesin and ezrin. Myosin phosphatase composed of the catalytic subunit and MBS showed phosphatase activity toward moesin, which was phosphorylated by Rho-kinase. The phosphatase activity was inhibited when MBS was phosphorylated by Rho-kinase. These results suggest that MBS is recruited with moesin to the plasma m...Continue Reading

References

Oct 1, 1992·Current Opinion in Cell Biology·S TsukitaS Yonemura
Sep 1, 1990·The Journal of Cell Biology·H F PatersonA Hall
Dec 1, 1989·Immunology Today·B F HaynesS M Denning
Jan 1, 1985·Annual Review of Pharmacology and Toxicology·K E Kamm, J T Stull
Aug 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R PakkanenA Vaheri
Jun 1, 1995·Trends in Biochemical Sciences·Y TakaiH Nakanishi
Mar 1, 1995·Molecular Biology of the Cell·K PestonjamaspE J Luna
Feb 1, 1995·Current Opinion in Cell Biology·K A Giuliano, D L Taylor
Nov 1, 1993·Zygote : the Biology of Gametes and Early Embryos·I MabuchiS Narumiya
Apr 15, 1994·Biochemical and Biophysical Research Communications·S OkuboD J Hartshorne
Feb 1, 1994·Current Opinion in Genetics & Development·C Nobes, A Hall
Jan 1, 1994·Experimental Cell Research·M R AmievaH Furthmayr
Jan 1, 1993·Advances in Immunology·J LesleyP W Kincade
Dec 18, 1995·FEBS Letters·P MadauleS Narumiya
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·M C GongA P Somlyo
Aug 23, 1996·The Journal of Biological Chemistry·M AmanoK Kaibuchi
Sep 24, 1996·Biochemical and Biophysical Research Communications·F NakamuraH Furthmayr
Jan 2, 1997·The EMBO Journal·D T DransfieldJ R Goldenring
Jan 23, 1997·Biochemical and Biophysical Research Communications·N InagakiM Inagaki

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Citations

Apr 12, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Hugo CeulemansMathieu Bollen
Jun 16, 2000·Advanced Drug Delivery Reviews·A M HopkinsA Nusrat
Nov 9, 2000·Biology of the Cell·S Louvet-Vallée
Feb 27, 1999·Current Opinion in Cell Biology·P Aspenström
Feb 27, 1999·Current Opinion in Cell Biology·A Bretscher
Jul 7, 2001·Trends in Biochemical Sciences·M Bollen
May 1, 1998·Trends in Cardiovascular Medicine·M AmanoK Kaibuchi
Aug 18, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·N NakamuraK Kaibuchi
Nov 9, 2004·The Journal of Biological Chemistry·Harn ShiueJerrold R Turner
Apr 13, 2004·Molecular Biology of the Cell·Arnold Lee, Jessica E Treisman
Oct 8, 2004·Molecular Biology of the Cell·Jacqueline MulderWouter H Moolenaar
Jul 1, 2005·Molecular Biology of the Cell·Kazuo Kurokawa, Michiyuki Matsuda
Jun 25, 2010·Molecular Biology of the Cell·Dagmar Neubueser, David R Hipfner
Feb 2, 1999·The EMBO Journal·F Sánchez-Madrid, M A del Pozo
Jun 29, 2000·Annual Review of Biochemistry·K KaibuchiM Amano
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·V P SahJ H Brown
Jul 1, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Csilla CsortosAlexander D Verin
May 14, 2009·The Journal of Clinical Investigation·A Valance WashingtonDaniel W McVicar
Oct 3, 2006·Respiratory Research·Luke J Janssen, Kieran Killian
Apr 4, 2009·Endocrinology·Rieko YodoiYukihiko Sugimoto
Sep 19, 2012·Cell Structure and Function·Michiro IizukaAkira Mizoguchi
Jan 8, 2011·Biological & Pharmaceutical Bulletin·Kenji SakamotoKunio Ishii
Jun 23, 2012·PloS One·Takako Kaneko-KawanoKenji Suzuki
Jun 27, 2009·Journal of the American Society of Nephrology : JASN·Francesca Di SoleOrson W Moe
Aug 31, 2001·Biochemical and Biophysical Research Communications·B LubecM Fountoulakis
Jun 30, 2000·Biochemical and Biophysical Research Communications·T KanekoK Kaibuchi
Nov 10, 2000·Biochemical and Biophysical Research Communications·T IzawaK Kaibuchi
Nov 26, 2002·Immunological Reviews·Patrick CullinanJanis K Burkhardt
Mar 9, 2005·The Journal of Cell Biology·Karin EhrenreiterManuela Baccarini
Jun 19, 2008·The Journal of Cell Biology·Hsiao-Hui Lee, Zee-Fen Chang
Apr 20, 1999·The Journal of Cell Biology·Y FukataK Kaibuchi
Sep 6, 2002·The Journal of Cell Biology·Emmanuelle MédinaAndré Le Bivic

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