Association of the Promoter Methylation and the rs12917 Polymorphism of MGMT with Formation of DNA Bulky Adducts and the Risk of Lung Cancer in Mexican Mestizo Population

DNA and Cell Biology
Ollin Celeste Martínez-RamírezJulieta Rubio

Abstract

O6-Methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs the DNA damage caused by the tobacco habit, and low activity of this enzyme has been associated with a risk of lung cancer (LC). Our objective was to determine the association of the promoter methylation and the rs12917 polymorphism of MGMT with formation of DNA bulky adducts and the risk of LC in the Mexican Mestizo population. In this study are included 431 subjects. High-resolution melting analysis was used to determine the polymorphism MGMT rs12917 and methylation levels. DNA bulky adducts were determined by 32P-postlabeling. Our results showed that MGMT rs12917 and higher levels of methylation in the MGMT promoter are associated with the risk of LC. The levels of adducts are related with the phe/phe genotype and, only in the cases group, with the hypermethylation (>50%) of MGMT; however, this last association was not statistically significant.

References

Oct 21, 1991·Nucleic Acids Research·D K Lahiri, J I Nurnberger
Mar 29, 2000·Environmental and Molecular Mutagenesis·D H PhillipsK W Turteltaub
Apr 18, 2000·Mutation Research·A E Pegg
Jan 24, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lili Liu, Stanton L Gerson
Jun 17, 2006·Current Opinion in Pharmacology·Ami Sabharwal, Mark R Middleton
Jun 17, 2006·Carcinogenesis·Jiali HanImmaculata De Vivo
Feb 10, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Janakiraman Subramanian, Ramaswamy Govindan
Feb 16, 2007·The New England Journal of Medicine·Megan P HitchinsRobyn L Ward
May 8, 2007·DNA Repair·Anthony E PeggNatalia A Loktionova
Mar 1, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shuguang LengSteven A Belinsky
Dec 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Diamantis SellisPhilipp W Messer
Jul 21, 2012·Wiener klinische Wochenschrift·Caroline A ThompsonGerald Haidinger
Oct 3, 2013·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Edith MolinaMaría E Gonsebatt
Jul 2, 2014·The Journal of Cell Biology·Michael EnsmingerMarkus Lӧbrich
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray
Apr 25, 2015·Environmental Pollution·Martha Estela Salcido-NeyoyClaudia M García-Cuellar

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Citations

Aug 16, 2019·The Journal of Gene Medicine·Heba F PashaAhmed M Yehia
Jun 7, 2021·Rheumatology International·Demet Kivanc, Selcuk Dasdemir

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Methods Mentioned

BETA
biopsy
genotyping
PCR

Software Mentioned

Statistical Package for Social Sciences ( SPSS - PC
Prism
GraphPad

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